安博维对兔心肌梗死后心功能及基质金属蛋白酶-3表达的影响

目的：研究兔心肌梗死后基质金属蛋白酶3（MMP-3）在心室重构和心衰形成中的变化及安博维对其的影响。方法：采用兔冠状动脉前降支结扎法建立兔心肌梗死模型。将28只新西兰兔随机分为伪手术组，心肌梗死组，药物干预组。手术前及手术后10周心超检测室间隔厚度（LVSd），左室后壁厚度（LVPWd），左室射血分数（EF）。用Western Blot法测定10周后心肌组织的基质金属蛋白酶3的含量。结果：手术后10周时，药物干预组LVSd，LVPWd明显低于心肌梗死组（P〈0．05）；左室射血分数明显高于心肌梗死组（P〈0．05），但低于伪手术组（P〈0．05）。心肌梗死后MMP-3表达升高，相对含量与EF呈负相关。结论：MMP-3参与了左室重构和心肌梗死后心衰的形成。安博维通过抑制MMP-3的表达，明显减轻心肌梗死后心肌肥厚，改善左室功能。

Effect of Aprovel Therapy to Heart Function and MMP-3 Expression after Acute Myocardial Infarction in Rabbits

Objective: To investigate the change of matrix metalloproteinases during the ventricular remodeling and heart failure induced after acute myocardial infarction in rabbits and the effect of Aprovel.Methods: Rabbits myocardial infarction model was established by permanent ligation of left anterior descending coronary artery.Twenty-eight New Zealand rabbits were randomized divided into 3 groups:sham group,infarcted group and drug treatment group.Pre-operation and 10 weeks after operation,the interventricular septum thickenss(IVSd),left ventricular posterior wall thickness(LVPWd),left ventricular ejection fraction(EF) were measured by echocardiography.Expression of MMP-3 protein in myocardium tissues was quantified by Western Blotting.Results: At 10th week,both IVSd and LVPWd had a significant decrease in drug treatment group compared with those of infarcted group(P<0.05).EF was obviously increased(P<0.05),but still lower than sham group(P<0.05).Expression of MMP-3 protein increased after myocardial infarction.The level of MMP-3 was correlated negatively with the EF.Conclusion: The alteration of MMP-3 contribute to the ventricular remodeling and heart failure induced after acute myocardial infarction.Aprovel can reduce myocardial hypertrophy,improve heart function by reducing the activity of MMP-3.