| 中国蝮蛇蛇毒中小肽SV-PP-3的分离及其抗血小板聚集作用 |
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| 引用本文： | 徐伟,吴少瑜,张嘉杰,李志琴,吴曙光. 中国蝮蛇蛇毒中小肽SV-PP-3的分离及其抗血小板聚集作用[J]. 南方医科大学学报, 2005, 25(4): 476-478 |
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| 作者姓名： | 徐伟 吴少瑜 张嘉杰 李志琴 吴曙光 |
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| 作者单位： | 南方医科大学药物研究所, 广东, 广州, 510515 |
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| 基金项目： | 收稿日期:2005-1-5。作者简介:徐伟(1971-),女,讲师,博士研究生,电话:020-61648167,E-mail:xuwei@fimmu.com通讯作者:吴曙光,教授,博士生导师,电话:020-61648166 |
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| 摘 要： | 目的 研究中国蝮蛇蛇毒中具有抑制血小板聚集作用的小肽。方法 采用低温离心、SephadexG-75凝胶层析、高效液相C-18反相层析从蛇毒中分离纯化小肽,并用血小板聚集仪测定分离的小肽在体外对磷酸腺苷(ADP)诱导的兔血小板聚集作用。结果 SV-PP-3是从中国蝮蛇蛇毒中分离出一种相对分子质量为1234.557的新的小肽。SV-PP-3在体外能显著抑制ADP诱导的兔血小板聚集,具有剂量依赖性。结论 SV-PP-3对ADP诱导的兔血小板聚集具有显著的抑制作用。
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| 关 键 词： | 蝮蛇 蛇毒液类 小分子肽 血小板聚集 |
| 文章编号： | 1000-2588(2005)04-0476-03 |
| 修稿时间： | 2005-01-05 |
Purification of a small peptide SV-PP-3 from the venom of A gkistrodon blomhoffii siniticus and its inhibition of platelet aggregation |
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| XU Wei,WU Shao-yu,ZHANG Jia-jie,LI Zhi-qin,WU Shu-guang. Purification of a small peptide SV-PP-3 from the venom of A gkistrodon blomhoffii siniticus and its inhibition of platelet aggregation[J]. Journal of Southern Medical University, 2005, 25(4): 476-478 |
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| Authors: | XU Wei WU Shao-yu ZHANG Jia-jie LI Zhi-qin WU Shu-guang |
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| Abstract: | Objective To investigate the inhibitory effect of the small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus on platelet aggregation. Methods The small peptide SV-PP-3 was isolated and purified by centrifugation at low temperature, sephadex G-75 column chromatography and reverse-phase (HPLC) on C-18 column. The platelet aggregation induced by adenosine diphosphate (ADP) was measured by LBY-NJ aggregometer. Results SV-PP-3 was purified from the venom of Agkistrodon blomhoffii siniticus, which inhibited the platelet ADP-induced platelet aggregation in a dose-dependent manner. Conclusion SV-PP-3 can inhibit ADP-Induced platelet aggregation. |
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| Keywords: | Agkistrodon halys siniticus snake venoms small peptide platelet aggregation |
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