芩丹胶囊对血管外膜成纤维细胞的作用机制

目的  探讨芩丹胶囊（QC）含药血清对动脉血管外膜成纤维细胞（AF）的作用机制。方法  采用组织贴块法体外原代培养大鼠胸主动脉成纤维细胞，MTT法观察大［750mg/（kg·d）］、小［150mg/（kg·d）］剂量的QC含药血清对转化生长因子β1 (TGF-β1)诱导的大鼠胸主动脉成纤维细胞的增殖的影响;实时定量PCR及western blot分别检测α-平滑肌肌动蛋白（α-SM actin）和Smad3的基因及蛋白表达情况。结果  大、小剂量QC含药血清呈剂量依赖性抑制TGF-β1诱导的AF的增殖（P＜0.05），且用药组α-SM actin和Smad3基因及蛋白的表达较单纯刺激组显著降低（P＜0.05）。结论  QC含药血清通过干扰TGF-β1/Smad信号转导通路抑制AF增殖和分化,从而改善和逆转动脉血管外膜重构。

Effect of Qindan Capsule on arterial adventitial fibroblasts

Objective  To investigate the effect and mechanism of the Qindan Capsule-containing serum on adventitial fibroblasts(AFs). Methods  AFs were cultured by tissue explant in vitro and induced by TGF-β1. The proliferation of AFs affected by the Qindan Capsule-containing serum with high［750mg/（kg·d）］ or low［150mg/（kg·d）］ dose was detected by MTT. Protein and mRNA expressions of α-SM actin and Smad3 were observed by Western-blot and Real-time PCR respectively. Results  Both high and low doses of the Qindan Capsule-containing serum could reduce proliferation of AFs induced by TGF-β1（P＜0.05） and inhibit expressions of mRNA and protein of α-SM actin and Smad3（P＜0.05）. Conclusion  The Qindan Capsule-containing serum has significant improved and reverse effect on aortic vascular remodeling. The mechanism may be related to inhibiting  proliferation and differentiation of AFs through the  TGF-β1/Smad signaling pathway.