|
|||||
|
|
| 碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球 | |
| 引用本文： | 杨琴,李琦,曾志磊,李傲,谢鹏,杨军,任俊伟.碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球[J].解剖学报,2010,41(3):339-343. |
| 作者姓名： | 杨琴 李琦 曾志磊 李傲 谢鹏 杨军 任俊伟 |
| 作者单位： | 1. 重庆医科大学附属第一医院神经内科,重庆,400016 2. 郑州市中心医院神经内科,郑州,450007 3. 重庆工学院生物工程学院,重庆,400050 |
| 基金项目： | 重庆市自然科学基金，重庆市教委科学基金 |
| 摘 要： | 目的观察碱性成纤维细胞生长因子(bFGF)和表皮生长因子(EGF)联合诱导骨髓基质细胞(MSCs)能否分化形成神经球。方法采用全骨髓法培养MSCs,bFGF和EGF联合诱导MSCs,倒置显微镜下观察分化细胞的形态,免疫荧光法和免疫印迹法鉴定分化细胞。结果 bFGF和EGF联合应用,可有效地诱导MSCs形成神经球。此神经球能传代并分化成神经元样细胞。免疫荧光和免疫印迹法均证实神经球表达nestin蛋白,神经球分化的细胞表达神经元、胶质细胞和少突胶质细胞标志性蛋白。结论 bFGF和EGF联合应用,可有效地诱导MSCs形成神经球。此神经球能自我更新并分化为神经元、胶质细胞和少突胶质细胞。 |
| 关 键 词： | 骨髓基质细胞 碱性成纤维细胞生长因子 表皮生长因子 神经球 分化 免疫荧光 免疫印迹法 大鼠 |
| 收稿时间： | 2009-3-13 |
| 修稿时间： | 2009-5-19 |
Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats |
|
| YANG Qin,LI Qi,ZENG Zhi-lei,LI Ao,XIE Peng,YANG Jun,REN Jun-wei.Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats[J].Acta Anatomica Sinica,2010,41(3):339-343. | |
| Authors: | YANG Qin LI Qi ZENG Zhi-lei LI Ao XIE Peng YANG Jun REN Jun-wei |
| Institution: | 1.Department of Neurology, the First Hospital Affiliated, Chongqing Medical University, Chongqing 400016, China;2.Department of Neurology, Zhengzhou Central Hospital, Zhengzhou 450007, China;3.College of Bioengineer, Chongqing Institute of Technology, Chongqing 400050, China |
| Abstract: | Objective To observe whether bone marrow stromal cells(MSCs) can be induced to form neurospheres by basic fibroblast growth factor(bFGF) combined with epidermal growth factor (EGF) in rats. Methods MSCs were cultured with whole bone morrow. Effect of bFGF and EGF, inducing MSCs to differentiated into neurospheres was studied by morphology, immunofluorescence, and Western blotting. Results MSCs can be effectively induced to form neurospheres by bFGF and EGF.The neurospheres can passage and differentiated into neural cells.Immunofluorescence and Western blotting showed these neurospheres expressed nestin protein. Cells differentiated from neurospheres expressed marker proteins of neurons, glial cells and oligodendrocytes. Conclusion The results indicate that MSCs can be effectively induced to form neurospheres by bFGF and EGF. The neurospheres may amplify and differentiate into neurons, glial cells and oligodendrocytes. |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《解剖学报》浏览原始摘要信息 | |