| 人红细胞的Ca~(2+)和Cd~(2+)运输 |
| |
| 引用本文： | 赵昇皓,张槐耀.人红细胞的Ca~(2+)和Cd~(2+)运输[J].徐州医学院学报,1988(1). |
| |
| 作者姓名： | 赵昇皓 张槐耀 |
| |
| 作者单位： | 徐州医学院生物化学研究室
(赵昇皓)，美国犹大儿童研究医院生化系田纳西大学医学中心(张槐耀) |
| |
| 摘 要： | 用人红细胞膜、翻膜小囊和完整红细胞研究了Cd~(2+)对Cd~(2+)运输的影响与Cd~(2+)-ATP酶活性的关系,以及细胞对Cd~(2+)的摄取和影响摄取的因素。Cd~(2+)抑制Cd~(2+)运输,与抑制Cd~(2+)-ATP酶具有相关性。Cd~(2+)极易进入细胞,一旦进入,难于排出。进入细胞的Cd~(2+)主要与胞浆和膜蛋白相结合。
|
| 关 键 词： | 红细胞 翻膜囊 钙 镉中毒 Ca~(2+)-ATP酶 钙调素 人类 |
Ca~(2 +) AND Cd~(2 +) TRANSPORT IN HUMAN ERYTHROCYTE |
| |
| Chao Shenghao.Ca~(2 +) AND Cd~(2 +) TRANSPORT IN HUMAN ERYTHROCYTE[J].Acta Academiae Medicinae Xuzhou,1988(1). |
| |
| Authors: | Chao Shenghao |
| |
| Abstract: | In this study, we investigated the effects of Cd2+ on the Ca2 + -ATPase of human erythrocyte membranes, the Ca2 + transport in inside-out vesicles, and the Cd2 + transport in intact erythrocytes. Cd2+ inhibited the Ca2 + -ATPase activity of human red blood cell membranes, probably through an effect on the catalytic site of the enzyme. The inhibition by Cd2+ could be reversed by thiols, such as dithiothretol and glutathionine. Cd2 + had an inhibitory effect on the Ca2 + uptake by inside-out membrane vesicles.This inhibitory effect correlated well with that on the Ca2 + -ATPase. In contrast with the marked impermeability to Ca2 + , the red cells were able to accumulate Cd2 + , which was accelerated by the calcium ionophore A 23187 and glucose. As most Cd ions entering the red cells were associated with cytosol and membrane bound proteins, the cations were released very slowly into the medium. |
| |
| Keywords: | erythrocyte inside-out vesicles calcium cadmium toxicity Ca~(2+) -ATPase calmodulin human |
| 本文献已被 CNKI 等数据库收录！ |
|
