TLR expression in human melanoma cells |
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Authors: | Saint-Jean Mélanie Knol Anne-Chantal Nguyen Jean-Michel Khammari Amir Dréno Brigitte |
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Institution: | Immuno-dermatology laboratory, Onco-dermatology unit, CIC biothérapie INSERM 0503, CHU H?tel-Dieu, 1 place A. Ricordeau, 44093 Nantes Cedex 1, France. |
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Abstract: | There is increasing evidence that melanoma cells express TLR2, -3, and -4. However, the expression of other TLRs by these cells still remains unknown. We investigated the expression patterns of TLR2, -3, -4, -7, -8 and -9 both on melanoma-invaded lymph nodes and on melanoma cell lines. TLR2, -3, -4, -7 and -9 mRNA expression was determined by quantitative RT-PCR. The TLR protein expression level was measured ex vivo by immunohistochemistry and in vitro by flow cytometry. Results: At the mRNA level, melanoma cells in vitro, and possibly ex vivo, expressed TLR2, -3, -4, -7 and -9. TLR2 and -4 protein expressions ex vivo were over 50%, contrasting with an absence of these 2 TLRs in vitro. On the contrary, TLR-3 and -8 proteins had a low expression ex vivo with a high expression in vitro. TLR-7 and -9 proteins were expressed ex vivo and in vitro. Our study demonstrates for the first time that melanoma cells express TLR7 and -8. |
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