| 非小细胞肺癌中PI3K/Akt信号通路通过Cdh1调控Skp2表达的机制研究 |
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| 引用本文： | 孙秀华,张洪开,李玉,于爱鸣.非小细胞肺癌中PI3K/Akt信号通路通过Cdh1调控Skp2表达的机制研究[J].山东医药,2011,51(12):30-32,118. |
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| 作者姓名： | 孙秀华 张洪开 李玉 于爱鸣 |
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| 作者单位： | 中国医科大学,沈阳,110001 |
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| 基金项目： | 辽宁省教育厅资助项目(2004D225) |
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| 摘 要： | 目的探讨非小细胞肺癌（NSCLC）中Cdc20同源蛋白1（Cdh1）参与磷脂酰肌醇三羟基激酶（PI3K）/Akt信号通路对S期激酶相关蛋白2（Skp2）表达调控的机制。方法体外培养NSCLC细胞系A549、LK2和H460,LY294002特异性阻断PI3K/Akt信号通路后,Western blot检测Skp2、Cdh1及p-Akt蛋白表达的变化,免疫荧光（IF）检测Cdh1在NSCLC中的定位变化。结果 LY294002处理后,与对照组相比3种细胞中Skp2蛋白表达和Akt磷酸化水平均降低（P〈0.01）,Cdh1在3种细胞的核内表达均增多。结论 NSCLC中PI3K/Akt信号通路抑制剂LY294002使Skp2蛋白表达下调与Cdh1由细胞质向细胞核转位有关。
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| 关 键 词： | 肺肿瘤 磷脂酰肌醇三羟基激酶 S期激酶相关蛋白2 Cdc20同源蛋白1 |
Regulative mechanism of PI3K/Akt signal pathway on Skp2 via Cdh1 in non-small cell lung cancer |
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| SUN Xiu-hua,ZHANG Hong-kai,LI Yu,YU Ai-ming.Regulative mechanism of PI3K/Akt signal pathway on Skp2 via Cdh1 in non-small cell lung cancer[J].Shandong Medical Journal,2011,51(12):30-32,118. |
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| Authors: | SUN Xiu-hua ZHANG Hong-kai LI Yu YU Ai-ming |
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| Institution: | SUN Xiu-hua,ZHANG Hong-kai,LI Yu,YU Ai-ming(China Medical University,Shenyang 110001,P.R.China) |
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| Abstract: | Objective To investigate the regulative mechanism of phosphatidylinositol 3 kinase(PI3K)/Akt signaling pathway on S-phase kinase-associated protein 2(Skp2) via Cdc20 homolog 1(Cdh1) in non-small cell lung cancer(NSCLC).Methods NSCLC cell lines A549,LK2 and H460 were cultured in vitro and treated with LY294002 to block the PI3K/Akt pathway.Western blot method was used to detect the expression of Skp2,Cdh1 and p-Akt proteins.Immunofluorescence(IF) analysis was used to examine the localization of Cdh1 in NSCLC... |
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| Keywords: | lung cancer phosphatidylinositol 3 kinase S-phase kinase-associated protein 2 Cdc20 homolog 1 |
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