Annexin a2对人乳腺癌MDA-MB-231增殖的影响及分子机制

目的研究Annexin a2的表达对人乳腺癌细胞MDA-MB-231增殖、细胞周期分布和克隆形成能力的影响,并探讨其可能的分子机制。方法采用小干扰RNA（siRNA）技术下调人乳腺癌细胞中Annexin a2基因的表达,利用MTT比色法、流式细胞术、平板克隆形成实验观察Annexin a2表达水平下降后乳腺癌细胞增殖、周期分布和克隆形成能力的变化;利用免疫共沉淀和免疫荧光分析在MDA-MB-231细胞中与Annexin a2相互作用的蛋白。结果 Western blot证实siRNA干扰后MDA-MB-231细胞中Annexin a2蛋白表达水平明显下降;同时细胞增殖和克隆形成能力显著下降,细胞G0/G1期比例增高,G2/M和S期比例下降;免疫共沉淀发现Annexin a2与信号转导和转录激活因子3（STAT3）存在相互作用。结论 Annexin a2蛋白水平下降可以明显抑制人乳腺癌细胞MDA-MB-231增殖和克隆形成能力,Annexin a2与STAT3相互作用有可能参与乳腺癌细胞的增殖调节。

Effects of Annexin a2 on the proliferation, colony formation and cell cycle of human breast cancer cell line MDA-MB-231 and the possible molecular mechanism

Objective To study the effects of Annexin a2 on the proliferation,colony formation and cell cycle of human breast cancer cell line MDA-MB-231 and to explore the possible molecular mechanism.Methods Small interfering RNA(siRNA) technology was used to knock down the expression of Annexin a2 in MDA-MB-231 cells and monoclonal cell strains were screened.The expression level of Annexin a2 was detected by Western blot;the activities of proliferation and colony formation were examined by MTT,the changes of cell cy...