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| rshCD_（40）L、IFN-γ对食管癌Eca109、Eca9706、TE13细胞增殖和凋亡的影响 | |
| 引用本文： | 吴广洲,沈振亚,刘国锋,张爱娟,吴雨岗. rshCD_（40）L、IFN-γ对食管癌Eca109、Eca9706、TE13细胞增殖和凋亡的影响[J]. 山东医药, 2011, 51(6): 23-25 |
| 作者姓名： | 吴广洲 沈振亚 刘国锋 张爱娟 吴雨岗 |
| 作者单位： | 1. 苏州大学附属第一人民医院,江苏苏州,215006 2. 盐城市第一人民医院 3. 苏州大学附属第三人民医院 |
| 摘 要： | 目的观察可溶性重组人CD40L（rshCD40L）、IFN-γ对食管癌Eca109、Eca 9706、TE13细胞增殖和凋亡的影响。方法取正常培养的食管鳞癌细胞株Eca109、Eca 9706、TE13,分别用PBS、100 U/ml IFN-γ、100 ng/ml rsh-CD40L、100 U/ml IFN-γ＋100 ng/ml rshCD40L培养,分别为A、B、C、D组。用MTT法测算各组细胞增殖抑制率,用TUNEL法检测细胞凋亡率。结果 C组Eca109、Eca9706、TE13细胞增殖抑制率分别为40.6%±4.2%、31.5%±5.7%、44.6%±6.7%,明显高于A、B组（P均〈0.05）;D组分别为56.7%±4.9%、41.2%±6.2%、51.6%±5.2%,均高于C组（P均〈0.05）。C组Eca109、Eca9706、TE13细胞凋亡率分别为33.6%±3.7%、30.5%±2.8%和37.6%±4.9%,明显高于A、B组（P均〈0.05）;D组分别为43.7%±4.7%、34.2%±5.1%、41.5%±5.7%,均高于C组（P均〈0.05）。结论 rshCD40L能促进食管癌Eca109、Eca9706、TE13细胞凋亡,并抑制其增殖。IFN-γ可增强这一作用。 |
| 关 键 词： | 食管肿瘤 食管癌 CD40 rshCD40L 干扰素-γ 细胞增殖 细胞凋亡 |
Expression of CD40 and growth-inhibitory activity of CD40 ligand in esophageal squamous cell carcinoma in virto |
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| WU Guang-zhou,SHEN Zhen-ya,LIU Guo-feng,ZHANG Ai-juan,WU Yu-gang. Expression of CD40 and growth-inhibitory activity of CD40 ligand in esophageal squamous cell carcinoma in virto[J]. Shandong Medical Journal, 2011, 51(6): 23-25 | |
| Authors: | WU Guang-zhou SHEN Zhen-ya LIU Guo-feng ZHANG Ai-juan WU Yu-gang |
| Affiliation: | WU Guang-zhou1,SHEN Zhen-ya,LIU Guo-feng,ZHANG Ai-juan,WU Yu-gang(1 The First Affiliated Hospital of Soochow University,Suzhou 215006,P.R.China) |
| Abstract: | Objective To explore the expression of CD40 on esophageal squamous cell lines,and evaluated the effect of recombinant soluble human CD40L(rshCD40L) on esophageal cell lines.Methods The expression of CD40 on esophageal cell lines was examined by flow cytometry.The growth-inhibitory activity of rshCD40L on esophageal cancer cell was examined by MTT assay and the proportion of apoptotic tumor cells was examined in the TUNEL assay.Results The expression of CD40 was elevated in 3 esophageal cell lines examined b... |
| Keywords: | CD40 rshCD40L |
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