非小细胞肺癌肿瘤相关巨噬细胞表型检测及临床意义

目的检测非小细胞肺癌（NSCLC）患者肿瘤相关巨噬细胞表型及其分布,探讨NSCLC患者预后与肿瘤相关巨噬细胞各表型的关系。方法收集47例有完整临床病理和随访资料的NSCLC标本,运用免疫组化方法检测CD68、TNF-α、TGF-β1在肿瘤间质及癌旁肺组织巨噬细胞表达,分析其与患者各项临床指标及预后的关系。结果 CD6＋8TNF-α＋M1型巨噬细胞在NSCLC癌组织低于癌旁正常肺组织（P〈0.05）,CD6＋8TNF-β1＋M2型巨噬细胞在NSCLC肿瘤间质高于癌旁正常肺组织（P〈0.05）;M1型及M2型巨噬细胞在肿瘤间质内的数量与临床分期相关（P〈0.05）;Kaplan-Meier生存曲线分析显示患者术后生存率随巨噬细胞TNF-α阳性表达而呈上升趋势,随TGF-β1阳性表达呈现下降趋势。结论 M1型巨噬细胞减少和M2型巨噬细胞增多与NSCLC患者的不良预后有关。

Clinical significance of tumor-associated macrophage phenotypes in non-small cell lung cancer

Objective To study the relationship between the tumor-associated macrophage phenotypes and the clinical prognosis of non-small cell lung cancer（NSCLC） patients.Methods Immunohistochemical staining for CD68,TNF-α and TGF-β1 were performed on paraffin embedded tissues from 47 NSCLC cases.The relationship between immunohistochemical staining results and the relative clinicopathologic data and prognosis of NSCLC patients were analyzed.Results CD＋68TNF-α＋ macrophage（M1） was significantly lower in NSCLC than that in tumor surrounding lung tissues（P〈0.05） and CD＋68TNF-β＋1 macrophage（M2） was significantly higher in NSCLC than that in tumor surrounding lung tissues（P〈0.05）.M1 and M2 macrophages expression were correlated with clinical stage（P〈0.05）.Kaplan-Meier analysis showed that up-regulating of CD＋68TNF-α＋ macrophage expression was associated with higher survival rate of patients after operation,up-regulating of CD＋68TNF-β＋1 macrophage expression was associated with lower survival rate of patients after operation.Conclusion M1 macrophage decreasing and M2 macrophage increasing were correlated with lower survival rate of NSCLC patients after operation.