硫酸酯化箬叶多糖抗HIV-1机制的初步研究

目的 探讨硫酸脂化箬液多糖（Ｓ－ＩＴＰＳ）抗ＨＩＶ的机制，为进一步开发该药提供理论依据。方法 于病毒接种前，接种同时及接种后分别在培养细胞（ＭＴ－４）中加入硫酸脂化箬叶多糖（Ｓ－ＩＴＰＳ），在Ｓ－ＩＴＰＳ存在或不存在的条件下培养１￣４周。终点以病毒半数感染量（ＴＣＩＤ５０法），细胞病变（ＣＰＥ），ＭＴＴ染色细胞保护率（ＭＴＴ法）及培养上清中的ｐ２４抗原滴度９ＥＬＩＳＡ法）作为评价指标，判断药效，分

Mechanismof Se-Indocalamus tessdatus polycassine(S-ITPS) agacnst human immunodeficiency virus type1(HIV-1)

OBJECTIVE: To study the mechanism of Se- Indochalamus tessdatus polycassine (S-ITPS) against HIV-1 and to provide a theoretical support for developing the anti-HIV drugs. METHODS: S-ITPS was added to MT4 cells before viral inoculation, at the same time of viral inoculation, then the cells were cultured with or without S-ITPS for 1-4 weeks, finally, cytopathic effect (CPE), MTT staining method for viable cells(MTT assay),p24 antigen titer (ELISA), or infectivity (TCID50 assay) of the cultural supernatants were used as markers to monitor the virus growth. RESULTS: S-ITPS can inhibit HIV-induced CPE (1C50 = l0microg/ml)and viral replication (1C50 = 156 microg/ml)in dose dependent manner. The virus infectivity in the presence of S-ITPS was greatly decreased than that of the control. The virus inhibition was enhanced under the presence of noncytotoxic drug concentration of <1250 microg/ml in cell culture, inhibition of viral replication by S-ITPS was 73.7%, 63.5%, 87.7% and 95.4% at week 1, week 2, week 3 and week 4 of cultured cells respectively. It can also inhibit absorption of HIV-1 to MT-4 cells, but no inhibition of HIV-1 was seen when MT-4 cells were pretreated with S-ITPS. Virus can be inactivated by the agent also. CONCLUSIONS: S-ITPS is a potent anti-HIV- 1 agent in MT-4 /HIV- 1 cultural system, at least two mechanisms were included inhibition both of HIV-1 absorption to MT-4 cells and viral replication in HIV-infected cells. S-ITPS inhibition of CPE is stronger than that of p24 antigen production.