Antigenic and genetic analyses of influenza B viruses\\ isolated in Lusaka, Zambia in 1999 |
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| Authors: | J Muyanga Y Matsuzaki K Sugawara K Kimura K Mizuta I Ndumba Y Muraki E Tsuchiya S Hongo F C Kasolo Y Numazaki K Nakamura |
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| Institution: | (1) Virology Laboratory, Department of Microbiology and Pathology, University Teaching Hospital, Lusaka, Zambia, ZM;(2) Department of Bacteriology, Yamagata University School of Medicine, Yamagata, Japan, JP;(3) Yamagata Prefectural Institute of Public Health, Yamagata, Japan, JP |
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| Abstract: | Summary. Previous studies of the hemagglutinin (HA) genes of various influenza B virus isolates demonstrated the existence of two
antigenically distinct virus lineages represented by B/Victoria/2/87 and B/Yamagata/16/88, respectively. Here, we investigated
the antigenic and genetic characteristics of influenza B viruses isolated from children living in Lusaka, Zambia between January
and May 1999. Antigenic analysis with chicken antiviral sera showed that all the Zambian isolates had the HA protein belonging
to B/Yamagata/16/88-related lineage. Furthermore, phylogenetic analyses of the eight RNA segments performed by using the total
or partial nucleotide sequences of the two representative Zambian strains (B/Lusaka/270/99 and B/Lusaka/432/99) as well as
the previously reported sequences suggested that the Zambian viruses are closely related to the recently circulating reassortants
represented by B/Shiga/T30/98 and B/Yamanashi/166/98 which acquired the genes coding for three polymerase proteins (PB2, PB1,
and PA), HA, nucleoprotein, and matrix protein from a B/Yamagata/16/88-like parent and the gene encoding nonstructural proteins
(NS1 and NS2) from a B/Guandong/8/93-like parent.
Accepted June 15, 2001 Received April 17, 2001 |
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