Altered arachidonic acid metabolism in platelets and aortas from selenium-deficient rats |
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| Affiliation: | 1. CNRS/Univ. Pau & Pays de l''Adour, Institut des Sciences Analytiques et de Physico-Chimie pour l''Environnement et les Matériaux (IPREM), UMR 5254, 64053 Pau, France;2. Andra, Research and Development Division, Parc de la Croix Blanche, 92298 Châtenay-Malabry Cedex, France;3. INRAE, BEF, F-54000 Nancy, France;4. IC2MP UMR 7285, Université de Poitiers, 86073 Poitiers Cedex 9, France;1. Abant Izzet Baysal University Faculty of Medicine, Department of General Surgery, Bolu, Turkey;2. Abant İzzet Baysal University Faculty of Medicine, Department of Clinical Microbiology, Bolu, Turkey;3. Abant İzzet Baysal University Faculty of Medicine, Department of Histology and Embryology, Bolu, Turkey;1. School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2252, Australia;2. Molecular Horizons, University of Wollongong, Wollongong, NSW 2252, Australia;3. Illawarra Health and Medical Research Institute, Wollongong, NSW 2252, Australia;4. Centre for Kidney Research, The Children''s Hospital at Westmead, Westmead, NSW, Australia |
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| Abstract: | Selenium (Se) deficiency results in decreased activity of the seleno-enzyme, glutathione peroxidase (GSHPx), an intracellular scavenger of hydroperoxides which may thereby influence the activity of various enzymes of arachidonic acid (20:4) metabolism in platelets and aortic tissue from rats. In vitro studies, performed with platelets isolated by gel-filtration, demonstrated that platelets from selenium-deficient (−Se) rats aggregated to a significantly greater extent when stimulated by ADP and collagen than those from selenium-supplemented (+Se) rats. Platelets from the −Se rats also produced more thromboxane B2 (TXB2) when stimulated by collagen than did those from +Se rats. In aortic tissue from −Se rats, a significant depression in the generation of prostacyclin (PGI2)-like material was demonstrated compared to the tissue from +Se rats. Type of dietary fat and age of the animals also influenced the production of PGI2-like activity in aortas from both +Se and −Se rats. Our results suggest that an imbalance in the production of two 20:4 metabolites — proaggregatory TXA2 and antiaggregatory PGI2 — could occur in response to certain physiological stresses when GSHPx activity is reduced by a selenium-deficient diet. |
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