持久饥饿对涡虫转氨酶、磷酸酶、蛋白水解酶和增殖细胞核抗原的影响

目的探讨持久饥饿对涡虫转氨酶、磷酸酶、蛋白水解酶和增殖细胞核抗原(PCNA)的影响。方法采用全自动生化分析仪测定转氨酶的活性变化,用复性电泳技术分析磷酸酶和蛋白水解酶的变化,用半定量RT-PCR技术分析PCNA mRNA的表达变化。结果饥饿过程中谷丙转氨酶和谷草转氨酶的活性显著升高,是对照水平的10倍以上,恢复喂食后逐渐降低到正常水平;碱性磷酸酶(ALP)的活性在饥饿过程中明显减弱,而酸性磷酸酶(ACP)活性却显著增强;受饥饿影响,140kD和40kD蛋白水解酶的活性均明显增加;然而饥饿过程中PCNA的表达没有明显变化,说明饥饿对该基因的影响很小。结论持久饥饿对涡虫的生理和生化代谢有重要影响,酶活性的变化反应了涡虫适应持久饥饿的能量代谢变化。

Effects of prolonged starvation on aminotransferases, phosphatases, proteinases and proliferating cell nuclear antigens in planarians

Objective To investigate the effects of prolonged starvation on aminotransferases, phosphatases, proteinaes and proliferating cell nuclear antigens (PCNA) in planarians. Methods Automated biochemistry analyzer was employed to determine the enzymes activities of aminotransferases, non-denatured electrophoresis (G-PAGE) was used to analyze enzymes activities of phosphatases and proteinaes, the semi-quantitative RT-PCR was employed to investigate the expressed changes of PCNA mRNA. Results The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) elevated apparently during starvation, 10-fold higher than that of the control level, but reduced to the normal level after re-fed; The activities of alkaline phosphatase (ALP) decreased during starvation, whereas the activities of acid phosphatase (ACP) increased apparently; In response to starvation, the activities of 140kD and 40kD proteinases increased drastically; However, no changes of the expression level of PCNA mRNA during starvation were observed, which indicated that starvation had little influences on this gene. Conclusion Prolonged starvation has greater effects on the physiology and biochemical metabolism of planarians, and the changes of enzyme activities reflecte the changes of energy metabolism of planarians in response to prolonged starvation. This findings provide a basis for further understanding the physiological mechanism that planarians are in adaptation to starvation.