定量分析卵巢癌细胞中p~（53）基因蛋白表达的意义

目的研究卵巢癌ｐ（５３）基因蛋白表达的量与ＤＮＡ倍体水平、细胞增殖活性及组织学分级的关系。方法采用流式免疫荧光技术对卵巢癌４２例的常规石蜡标本单细胞悬液行ＤＮＡ含量和ｐ（５３）蛋白单克隆抗体免疫荧光标记定量测定。结果４２倒卵巢癌的异倍体率为７６．２％（３２／４２）。细胞增殖指数（ＰＩ）为３１．２４±５．３６。ｐ（５３）蛋白表达的阳性率为５９．５％（２５／４２），其表达的量与ＤＮＡ倍体水平、组织学分级及ＰＩ均相关（Ｐ＜０．０５，Ｐ＜０．０１），ＤＮＡ异倍体率与组织学分级亦有关（Ｐ＜０．０５）。卵巢癌ｐ（５３）蛋白表达阳性的ＤＮＡ含量、ＰＩ值均显著高于阴性者；组织学分化越差，ｐ（５３）蛋白表达阳性率越高。结论ｐ（５３）蛋白表达阳性可作为反映卵巢癌恶性程度的重要指标。

The significance of quantitative analysis of p￣(53) gene protein expression in ovarian carcinoma

To study the relationship among p53 gene protein expression , content of DNA ploid ,cellular proliferative activity and histopathological grade in routinely processed tisseue of ovarian carcinomas.Methods Flow cytometry and immunoflurescence staining technique were used to determine the p53 gene protein expression and DNA contents in 42 cases of ovarian carcinomas.Results Among the 42 cases of ovarian carcinomas, the aneuploidy rate, proliferative index(PI) and the positive rate of p53 gene protein expression were 76. 2%, 32. 24 ±5. 36 and 59. 5%,respectively. p53 gene protein expression was assaociated with DNA ploid, histopathological grade and PI. The DNA content and PI were higher in cases of p53 positive expression than those in negative ones.The poorer histological differentiaion was found to be related with higher p53 positive rate.Conclusion Positive expression of p53gene protein can be used as an important objective parameter reflecting the malignancy of ovarian carcinoma.