丹参水提液对染镉大鼠肾损伤的干预作用

目的探讨丹参水提液对染镉大鼠肾损伤的干预作用及其可能的作用机制。方法健康Wistar大鼠,雌雄各20只,鼠龄6周。适应性喂养1周,随机分组,空白组8只,模型组32只,空白组腹腔注射生理盐水5 m L/(kg·d),模型组以2.8 mg/(kg·d)氯化镉溶液腹腔注射。连续造模4周,观察大鼠一般情况,检测大鼠肾功能指标、肾脏组织病理改变。造模成功后,将模型组随机分为给药组、阴性、阳性对照组及空白对照组。给药组[丹参水提液高剂量2.7 g/(kg·d)、丹参水提液低剂量1.35 g/(kg·d)]灌胃,阳性对照组亚硒酸钠溶液0.05 mg/(kg·d)灌胃,阴性及空白对照组给予同体积生理盐水2.7 m L/(kg·d)。各组治疗10周,实验终末收集血清、尿液,并留取肾脏组织,待检测。结果丹参水提液低、高剂量组24 h尿量低于阴性对照组,而血清超氧化物歧化酶(SOD)明显升高,丙二醛(MDA)显著降低,总抗氧化能力(T-AOC)增强明显,血尿素氮、血清肌酐含量降低,尿蛋白(β2-MG)含量下降。病理切片可见阴性对照组肾脏近曲小管上皮细胞浑浊、肿胀变性、坏死;丹参水提液低、高剂量组血清、皮质镉含量低于阴性对照组;丹参水提液低、高剂量组凋亡率低于阴性对照组。结论丹参水提液能加速体内镉代谢,可有效干预镉致肾损伤,有助于延缓慢性肾功能衰竭,改善肾功能,保护肾脏。

Effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium

Objective To explore effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium and its possible mechanism.Methods Twenty male and female healthy Wistar rats,respectively,at age of 6 weeks were randomly divided into blank group (n =8),and model group (n =32) after adaptive feeding for 1 week,given intraperitoneal injection of saline for 5 mL/(kg · d) and intraperitoneal injection of cadmium chloride solution for 2.8 mg/(kg · d).Continuously building for 4 weeks,the general situation of rats was observed,and the rat kidney function and kidney tissue pathological changes were detected.After successful molding,the model group was randomly divided into medicine group,negative and positive and blank control groups.The medicine group was given Salvia miltiorrhiza water extract of high dose of 2.7g/(kg · d),Danshen aqueous extract of low dose 1.35 g/(kg · d) by gavage,positive control group was given sodium selenite for 0.05 mg/kg · d by gavage,the negative and blank control groups were given the same mount of saline for 2.7 mL/(kg · d).After 10 weeks of treatment,serun and urine was collected,and kidney tissue was to be detected.Results Salvia miltiorrhiza water extraction liquid of low and high dose group had less 24 h urine volume than negative control group,and serum superoxide dismutases (SOD) increased significantly,Malondialdehyde (MDA) decreased significantly,total antioxidant capacity (T-AOC) increased significantly,blood urea nitrogen,urinary beta β2-MG content and serum creatinine levels was decreased.Pathological section revealed that renal proximal convoluted tubules epithelial cells was turbid,swelling,degeneration and necrosis in the negative control group.Salvia miltiorrhiza water extraction liquid of low and high dose group had lower serum cortical cadmium content than the negative control group,Salvia miltiorrhiza water extraction liquid of low and high dose group had lower apoptosis rate than the negative control group.Conclusion The salvia miltiorrhiza water solution can accelerate the metabolism of cadmium,and effectively interfere with cadmium induced renal injury,delay chronic renal failure,and protect the kidney.