| 镉、锌与金属硫蛋白抗体对金属硫蛋白的竞争结合 |
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| 引用本文： | 李慧祺,梁艺怀,雷立健,金泰廙.镉、锌与金属硫蛋白抗体对金属硫蛋白的竞争结合[J].劳动医学,2009(2):118-120. |
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| 作者姓名： | 李慧祺 梁艺怀 雷立健 金泰廙 |
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| 作者单位： | 复旦大学公共卫生学院劳动卫生学教研室; |
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| 摘 要： | 目的]通过镉、锌与金属硫蛋白抗体（MT-Ab）对金属硫蛋白（MT）的竞争结合，探讨MT-Ab在镉致肾损伤过程中的作用机制。方法]根据不同的实验处理分组，每组包含0、5、10、20、40mg／mL 5个不同实验浓度亚组。采用竞争ELISA法，按波长490nm处的光密度值判断竞争结合的程度。结果]实验所用包被MT质量浓度为1μg／mL，MT-Ab浓度为32U。随着实验镉、锌离子浓度增加，光密度值下降。结论]镉、锌与MT-Ab对MT的结合存在着竞争作用，MT-Ab可使CdMT解离产生游离镉离子，从而导致肾损伤效应。
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| 关 键 词： | 镉 锌 金属硫蛋白 金属硫蛋白抗体 竞争结合 |
Competitive Combination with Metallothionein between Metallothionein Antibody and Ions of Cadmium and Zinc |
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| LI Hui-qi,LIANG Yi-huai,LEI Li-jian,JIN Tai-yi.Competitive Combination with Metallothionein between Metallothionein Antibody and Ions of Cadmium and Zinc[J].Journal of Labour Medicine,2009(2):118-120. |
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| Authors: | LI Hui-qi LIANG Yi-huai LEI Li-jian JIN Tai-yi |
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| Institution: | Department of Occupational Health and Toxicology;School of Public Health;Fudan University;Shanghai 200032;China |
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| Abstract: | Objective] To discuss the mechanism of metallothionein antibody(MT-Ab)effecting cadmium induced kidney injury by exploring the competitive combination with metallothionein(MT)between MT-Ab and ions of cadmium and zinc.Methods] Groups were divided according to different treatments.Every group contained 5 different dose subgroups,namely 0,5,10,20 and 40 mg/mL respectively.Competitive enzyme linked immuno-sorbent assay(ELISA)was adopted.The extent of competitive combination was determined by photospectrometr... |
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| Keywords: | cadmium zinc metallothionein metallothionein antibody competitive combination |
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