| 人参总皂甙对小鼠胚胎发育的影响 |
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| 引用本文： | 柳鹏,殷惠军,张召锋,韩静,李丽,陈可冀,李勇. 人参总皂甙对小鼠胚胎发育的影响[J]. 中国生育健康杂志, 2006, 17(4): 230-232 |
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| 作者姓名： | 柳鹏 殷惠军 张召锋 韩静 李丽 陈可冀 李勇 |
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| 作者单位： | 1. 100083,北京,北京大学公共卫生学院营养与食品卫生学系
2. 中国中医研究院西苑医院 |
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| 基金项目： | 北京市自然科学基金资助项目(No.7044078) |
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| 摘 要： | 目的探讨人参总皂甙对体外培养的小鼠胚胎发育的影响。方法采用植入后全胚胎培养模型,将8.5d龄的ICR小鼠胚胎在含有不同浓度人参总皂甙的大鼠即刻离心血清中旋转培养48h(人参总皂甙终浓度分别为0,5,15,30,40,50mg/L),观察人参总皂甙对小鼠胚胎生长发育和组织器官形态分化的影响。结果随着人参总皂甙浓度的增加,反映胚胎生长发育的指标得分和胚胎总形态学得分无明显变化,各剂量组与对照组相比,差异无统计学意义(P>0.05)。结论人参总皂甙浓度达到50mg/L时,未见对体外培养的小鼠胚胎有毒性作用。
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| 关 键 词： | 全胚胎培养 人参总皂甙 小鼠 |
| 收稿时间： | 2006-06-02 |
| 修稿时间： | 2006-06-02 |
Study on the effects of ginsenosides on embryonic development by mouse whole embryo culture |
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| LIU Peng, YIN Huijun, ZHANG Zhaofeng,et al.. Study on the effects of ginsenosides on embryonic development by mouse whole embryo culture[J]. Chinese JOurnal of Reproductive Health, 2006, 17(4): 230-232 |
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| Authors: | LIU Peng YIN Huijun ZHANG Zhaofeng et al. |
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| Affiliation: | LIU Peng, YIN Huijun, ZHANG Zhaofeng, et al. |
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| Abstract: | Objective To examine the effects of ginsenosides on development of mouse embryos cultured in vitro. Methods Whole embryo culture system was used in this research. Early somite stage (GD 8.5) mouse embryos were explanted and cultured in rat serum medium (immediately centrifuged serum, ICS) with ginsenosides (0,5,15,30,40,50 mg/L) for 48 h to evaluate the embryonic development and morphological differentiation. Results With the concentration of ginsenosides rising, there were not significant changes in the growth and development score and total morphological score among groups. Conclusion At the concentration up to 50/L, ginsenosides didn't appear toxicity on mouse embryos yet. |
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| Keywords: | Ginsenosides Mouse Whole embryo culture |
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