超声辐照对主动脉平滑肌细胞增殖的抑制作用

目的　观察不同剂量的超声辐照对牛主动脉平滑肌细胞 (CASMC)增殖的影响 ,寻找出抑制细胞增殖的最佳辐照剂量 ,为临床治疗再狭窄提供理论依据。方法　体外培养的牛主动脉平滑肌细胞传至 3～ 8代 ,分为 4组 :第 1组为对照组 ;第 2组为超声辐照组 ;第 3组为血管紧张素Ⅱ (ATⅡ )组 ;第 4组为超声辐照+ATⅡ组。在 2 4孔和 96孔培养板上分别进行超声辐照 ,用细胞计数法、MTT法、3 H -TdR掺入法协同检测细胞增殖程度 ,采用多因素分析法逐步寻找出超声抑制细胞增殖的最佳辐照剂量。结果　 2 .2MHz,0 .5W /cm2 ,6 0s的超声剂量可以抑制CASMC增殖 ,抑制率达到 2 0 %～ 30 % (P <0 .0 1)。随着声强提高 ,辐照时间延长 ,对细胞的抑制率亦增高。在相同频率下 ,作用能量达到 1.0W /cm2 ,6 0s时 ,对细胞开始产生杀伤作用。结论　一定频率和剂量的超声辐照可以抑制CASMC增殖。

Ultrasound irradiation prevents the proliferation of calf aortic smooth muscle cells

Objective:To investigate the effect of ultrasound irradiation on the proliferation of calf vascular smooth muscle cells and find out the best irradiation dose to inhibit the proliferation.Methods:Cultured calf aortic smooth muscle cells(CASMC)were divided into 4 groups. The first acted as control group;the second was exposed to ultrasound which varied in irradiation dose; the third was given angiotensin Ⅱ ;the fourth was intervened by angiotensin Ⅱ and ultrasound irradiation. Cell counting, MTT, 3H-TdR incorporation were used to determine the extent of inhibition.Results:Ultrasound irradiation for 60 s at 2.2 MHz,0.5 W/cm2 could inhibit the proliferation of VSMCs and the rate was 20%～30% or so (P<0.01) .As the intensity increased, the time prolonged, the rate increased too. Ultrasound irradiation for 60 s at 2.2 MHz, 1 W/cm2 could kill them.Conclusions:Ultrasound irradiation at a certain frequency and dose can inhibit the CASMC proliferation induced by ATⅡ.