二苯乙烯类化合物E1的降糖作用及其机制的初步研究

目的　观察灌胃给予二苯乙烯类化合物E1对实验性非胰岛素依赖性糖尿病 (NIDDM )大鼠的降糖作用。方法　通过尾静脉注射小剂量链佐菌素 (streptozotocin ,STZ)来损伤大鼠胰岛细胞造成其糖耐量异常 ,继而喂以高糖 -高脂饲料来诱发动物肥胖形成的Ⅱ型糖尿病大鼠模型。结果　灌胃给予二苯乙烯类化合物E1能明显降低NIDDM大鼠空腹血糖、葡萄糖耐量实验 (OGTT)各时间点的血糖值 ;二苯乙烯类化合物E1.0 1mmol·L-1及 0 0 0 1mmol·L-1对βTC3 细胞无刺激其分泌胰岛素作用。结论　二苯乙烯类化合物E1的降糖作用并非通过刺激胰岛素的分泌 ,而可能是通过增加胰岛素作用效率

The glucose-lowering effect of stilbenoids E1 and mechanism

AIM To observe the glucose-lowering effect of orally administered stilbenoid E1 on rats with experimental non insulin dependent diabetes mellitus(NIDDM). METHODS The rat model of NIDDM was established by tail vein injection of a low dose of freshly prepared streptozotocin(STZ, 0 1 mmol·kg -1 ) which can injure the islet cells and cause impairment of glucose tolerance.Then, NIDDM rats were fed with high glucose high fat diets in order to make them obese. RESULTS stilbenoid E1 could significantly lower the fasting blood glucose and blood glucose levels of 60 min and 120 min in oral glucose tolerance test(OGTT) in NIDDM rats. However, stilbenoid E1 (0 1 mmol·L -1 and 0 001 mmol·L -1 ) in buffer with or without glucose could not stimulate βTC 3 cells to secret insulin. CONCLUSION stilbenoid E1 has a great glucose lowering effect by means of improving the effectiveness of insulin, but not by stimulating the islet cells to secret insulin.