survivin基因在膀胱移行细胞癌中的表达及其意义

目的: 探讨survivin基因在膀胱移行细胞癌(transitional cell carcinoma of bladder,TCCB)发生、发展和复发中的作用。方法: 对53例膀胱移行细胞癌和10例正常膀胱黏膜应用原位杂交方法检测survivin mRNA的表达。结果: survivin mRNA在正常的膀胱黏膜中无表达,在TCCB中的阳性表达率为71.7%,两者差异有统计学意义(P<0.005);survivin mRNA在TCCB中的阳性表达率随TCCB的病理分级、临床分期升高而增高,在G₁、G₂和G₃级TCCB中的阳性表达率分别是50.0%、68.2%和94.1%,差异有统计学意义(P<0.005);在表浅性和浸润性TCCB中的阳性表达率分别为50.0%、92.6%,阳性表达率两者差异有统计学意义(P<0.01);在初发和复发TCCB中的阳性表达率分别为66.7%、85.7%,两者阳性表达率差异有统计学意义(P<0.05)。结论: survivin mRNA高水平表达在TCCB的发生、发展和复发中发挥了重要作用。

Expression and clinicopathologic significance of surviving mRNA in transitional cell carcinoma of bladder

Objective:To investigate the expression of survivin mRNA and to evaluate its effects on the tumorigenesis,progression and recurrence in transitional cell carcinoma of bladder(TCCB).Methods:In 53 cases of TCCBs and 10 cases of normal bladder mucosas,the expression level of survivin mRNA was detected by in situ hybridization(ISH).Results:The expression positive rate of survivin mRNA in TCCB(71.7%) was significantly higher than that in 10 normal bladder tissues(P<0.005).The expression level of surviving mRNA was elevated as the grade and stage increased in TCCB.The positive rates in grade G1,G2,G3 were 50.0%,68.2% and 94.1%(P<0.005),50.0% and 92.6% in stage of Ta-T1,T2-T4,respectively.All the data in above groups were statistically significant(P<0.01);and in primary and recurrent cases were 66.7% and 85.7% and the data was statistically significant(P<0.05).Conclusions:The higher expression of survivin mRNA may play an important role in the tumorignesis,development and recurrence of TCCB.