Transdermal iontophoretic delivery and degradation of vasopressin across human cadaver skin |
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| Institution: | 1. Institute of Biochemistry & Biophysics, University of Tehran, Tehran, Iran;2. Department of Radiobiology, Tehran University of Medical Sciences, Tehran, Iran;1. Interdisciplinary Materials Research Center, School of Materials Science and Engineering, Tongji Univ, 4800 Caoan Rd, Shanghai, 201804, China;2. Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education, School of Materials Science and Engineering, Tongji Univ, 4800 Caoan Rd, Shanghai, 201804, China;3. Key Laboratory of Radiation Physics and Technology, Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, 610064, China;1. Department of Marine Biology, Tarbiat Modares University, Mazandaran, Noor, Iran;2. Iranian Fisheries Research Organization (IFRO), No. 325, Fatemi Ave., Tehran, Iran;3. Fisheries Department, Faculty of Marine Natural Resources, Khoramshahr Marine Science and Technology University, Iran;4. IRTA, Centre de Sant Carles de la Rápita (IRTA-SCR), Unitat de Cultius Experimentals, Crta. del Poble Nou Km 5.5, Sant Carles de la Rápita, Spain;1. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran;2. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran;3. Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;4. Department of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University;5. Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;6. Nanotechnology & Regenerative Medicine Commercialization Centre (Ltd), London BioScience Innovation Centre, London, United Kingdom;7. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran;8. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;1. Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada;2. Department of Radiation Oncology, University of Toronto, Toronto, ON, Canada;1. Department of Pharmaceutics, K.L.E. University''s College of Pharmacy, Bengaluru, India;2. Department of Pharmacology, K.L.E. University''s College of Pharmacy, Bengaluru, India;3. Department of Pharmaceutics, K.L.E. University''s College of Pharmacy, Belgavi, India |
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| Abstract: | Transdermal iontophoretic transport and degradation of a peptide, vasopressin, across human cadaver skin, was investigated. Modified Valia-Chien cells were supplied with 0.5 mA/cm2 current density via silver/silver chloride electrodes from a Scepter® power supply. Vasopressin (0.25 mg/ml) spiked with 3Hvasopressin was transported across skin with anode in donor. Samples were analyzed by HPLC using a radio-chromatography detector. Degradation of vasopressin in contact with intact skin and in skin homogenates was also studied. Greater degradation was observed in receptor where the peptide was in contact with the dermal side of the skin. The cumulative amounts of intact vasopressin permeated during 8 h of iontophoretic transport was 15.37 (±5.31; n = 3) μg/cm2, which corresponds to only about 1% permeation. Of the total radioactivity permeated, only about 40% was intact vasopressin by 12 h. Several degradation peaks could be seen in the chromatogram. No intact vasopressin was found to permeate under passive conditions. The enzymatic activity of cadaver skin is likely to be less than that expected in an in vivo drug delivery situation. Therefore, delivery to patients would be even less than that predicted by this study. |
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