The effect of Lithium on endothelial-dependent relaxation in rat isolated aorta |
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| Affiliation: | 1. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran;3. International Campus, Tehran University of Medical Sciences, Tehran, Iran;4. Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Science, University of Manitoba, Winnipeg, MB, Canada;1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran;3. Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran;4. Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran;3. Department of Molecular Pathology, University of Queensland, UQ Center for Clinical Research, Queensland, Australia;4. Department of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Biochemistry and Medical Genetics, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada;6. Department of Pathology, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;7. Hematology/Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | - 1.1. In endothelium-containing rings of rat aorta, precontracted by phenylephrine, addition of acetylcholine (Ach), resulted in a concentration-dependent relaxation through the release of endothelial dependent relaxing factors, including nitric oxide (IC50 = 8.41 μM).
- 2.2. Pretreatment of the tissues with 20 μM indomethacin, significantly decreased the relaxation.
- 3.3. Preincubation of the preparations in medium solution in which sodium has been partially replaced by 0.5 mM lithium, significantly reduced Ach-induced endothelial dependent relaxation (EDR).
- 4.4. Lithium (2 mM) in medium, significantly increased Ach-induced relaxation.
- 5.5. As is shown in this study, lithium has two opposite actions on EDR, with the dose of 0.5 mM inhibiting, while the dose of 2 mM potentiates EDR. Thus it seems that the action of lithium on EDR is mediated through two separate mechanisms.
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