In vitro evaluation of drug release kinetics from liposomes by fractional dialysis |
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| Affiliation: | 1. Istanbul University, Istanbul Faculty of Medicine, Department of Physiology, Istanbul, Turkey;2. Erciyes University, Education Faculty, Department of Sience Education, Kayseri, Turkey;3. Istanbul University, Istanbul Faculty of Medicine, Department of Hematology, Istanbul, Turkey;1. Al-Nahrain University, College of Biotechnology, Dep. of Molecular and Medical Biotechnology, Baghdad, Iraq;2. Al-Nahrain University, College of Engineering, Dep. of Biomedical Engineering, Baghdad, Iraq;3. Al-Farahidi University, College of Medical Technology, Dep. of Medical Laboratory Techniques, Baghdad, Iraq;4. Al-Nahrain University, College of Biotechnology, Dep. of Molecular and Medical Biotechnology, Baghdad, Iraq |
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| Abstract: | A new dialysis method was designed with the purpose of studying drug release rate from liposomes. The liposomes were diluted directly in the continuous phase and dialysed against a small volume of buffer. The dialysate was changed at 10 min intervals and the amount of carboxyfluorescein released was calculated from a standard curve. To evaluate the new method, parallel measurements were recorded with the traditional carboxyfluorescein assay, which is based on direct measurements on liposomes containing a self-quenching concentration. The new method is called fractional dialysis, and is shown to have sensitivity similar to that of the carboxyfluorescein assay. In certain cases the fractional dialysis method gives a more accurate release profile. It is suggested that fractional dialysis can be a useful alternative to other techniques for recording the in vitro release characteristics of specific drugs from liposomes or other colloidal carriers. |
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