Evaluation of a commercially available ELISA assay for detection of Giardia lamblia in fecal specimens |
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| Affiliation: | 1. From Associated Regional and University Pathologists, University of Utah Medical School, Salt Lake City, Utah, USA;2. the Department of Pathology, University of Utah Medical School, Salt Lake City, Utah, USA;1. Department of Otolaryngology, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China;2. Institute of Translational Medicine, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China;3. School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, 325003, China;4. Jiangsu University Health Science Center, Zhenjiang, Jiangsu Province, 212001, China;1. Unipampa Federal University, Uruguaiana, Rio Grande do Sul, Brazil;2. Division of Allergy and Clinical Immunology, Federal University of São Paulo, São Paulo, Brazil;3. Department of Pediatrics, Federal University of Paraná, Curitiba, Paraná, Brazil;4. Dr Avelino L. Castelán Pediatric Hospital, Chaco, Argentina;5. Hospital Universitario Metropolitano, Barranquilla, Colombia;6. Hospital Universitario General Calixto García, Havana, Cuba;7. Centro de Asma y Alergia, Tegucigalpa, Honduras;8. Centro Médico San Angel, Xalapa, Mexico;9. Clínica de Alergia y Asma, Asuncion, Paraguay;10. Centro de Medicina Avanzada Dr. Abel González, Santo Domingo, Dominican Republic;11. Hospital Pediatrico Pereira Rossell, Montevideo, Uruguay;12. Clínica Regional del Este, Córdoba, Argentina;13. Centro Nacional de Biopreparados, Havana, Cuba;14. Centro de Pesquisa: Consultorios del médico de familia, Havana, Cuba;15. Hospital Universitario Infantil Sur, Santiago de Cuba, Cuba;p. Allergy Service of the Hospital ISSSTE, Veracruz, Mexico;q. Universidad Nacional Del Este, Asuncion, Paraguay;r. ABC Foundation School of Medicine, Santo André, Brazil;1. Sisli Etfal Education and Research Hospital, Department of Pediatric Immunology and Allergy, Istanbul, Turkey;2. Bakırkoy Dr. Sadi Konuk Education and Research Hospital, Department of Pediatric Immunology and Allergy, Istanbul, Turkey;3. Division of Immunology and Allergy, Department of Chest Diseases, School of Medicine, Hacettepe University, Ankara, Turkey;4. International Hospital, Yesilkoy, Istanbul, Turkey;1. Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, USA;2. Department of Environmental Health Sciences, School of Public Health, University of California Los Angeles, Los Angeles, CA, USA |
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| Abstract: | A total of 417 fecal samples preserved in 10% buffered formalin and PVA were submitted to a commercial microbiology laboratory only for the detection of Giardia lamblia. Results from fecal specimens collected from 411 patients with gastrointestinal symptoms were compared using the following methods: (a) standard Ova & Parasite (O&P) concentration; (b) Alexon's ProspecT/Giardia enzyme-linked immunosorbent assay (ELISA) test, and (c) Meridian's Direct Fluorescent Antibody (DFA) Stain. In the 29 specimens in which G. lamblia was detected, 10 were O&P, DFA and ELISA positive, 17 were only ELISA positive and two were only Ova & Parasite and Direct Fluorescent Antibody positive. Of the 29 positive specimens, 22 were confirmed as true positives. The ELISA sensitivity was 91% and the specificity was 98%. The expense associated with these methods to detect the presence of Giardia is $11.00, $8.95, and $12.80, respectively. In symptomatic patients, the ProspecT/Giardia ELISA is a cost-effective, rapid, and sensitive method for detecting the presence of G. lamblia in fecal specimens. |
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