The enantiomeric separation of metipranolol and desacetylmetipranolol on a cellulose tris-3,5-dimethylphenyl-carbamate chiral stationary phase |
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| Affiliation: | 1. Analytical Development, Chauvin Pharmaceuticals, Harold Hill, Romford, Essex RM3 8SL, UK;2. Division of Chemical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK;1. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA;2. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;3. Ludwig Center for Molecular Oncology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;1. Department of Internal Medicine III, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany;2. RCI Regensburg Center for Interventional Immunology, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany;1. Cancer Drug Resistance Laboratory, Department of Life Science, National Institute of Technology Rourkela, Odisha, 769008, India;2. Department of Comparative Biosciences and Carle Illinois College of Medicine and Cancer Centre of Illinois, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States;3. Department of Bioresources and Food Science, Konkuk University, Seoul, 05029, South Korea |
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| Abstract: | A high-performance liquid chromatographic (HPLC) method is described for direct separation of the enantiomers of metipranolol and its principal degradation product and main metabolite, desacetylmetipranolol. Separations are performed on a chiral stationary phase of cellulose tris-3,5-dimethylphenyl carbamate (Chiralcel OD), with hexane-propan-2-ol-diethylamine elution and UV detection at 278 nm. The method affords identification and determination of the optical purity of the bulk drug and its formulated ophthalmic products. |
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