Oil-induced arthritis in da rats: Tissue distribution of arthritogenic 14C-labelled hexadecane |
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| Institution: | 1. Department of Clinical Immunology, University Hospital, Uppsala University, Uppsala, Sweden;2. Department of Pharmacology, Kabi-Pharmacia AB, Uppsala University, Uppsala, Sweden;4. Department of Pharmaceutical Biosciences, Toxicology, Uppsala University, Uppsala, Sweden;3. Department of Rheumatology, Karolinska Hospital, Stockholm, Sweden;1. Research Laboratory of Dendrimers and Nanopolymers, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran;2. Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Science, Tabriz, Iran;1. Laboratory of Reproductive and Developmental Biology, Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810 Japan;2. Laboratory of Reproductive and Developmental Biology, Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan;1. Enteric NeuroScience Program, Mayo Clinic School of Medicine, Rochester, Minnesota;2. Department of Integrative Physiology and Pharmacology, Liberty University College of Osteopathic Medicine, Lynchburg, Virginia;1. Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO, United States;2. Department of Biomedical Engineering, Washington University, St. Louis, MO, United States;1. Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan;2. National Museum of Marine Biology and Aquarium, Pingtung 944401, Taiwan;3. Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333008, Taiwan;4. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan;5. Clinical Immunology Laboratory, Department of Medical Research, E-Da Hospital, Kaohsiung 824005, Taiwan;6. Instrumentation Center, National Taiwan University, Taipei 106319, Taiwan;7. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804201, Taiwan;8. Institute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung 804201, Taiwan;9. Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333008, Taiwan;10. Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan;11. Research Center for Chinese Herbal Medicine, Graduate Institute of Healthy Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333324, Taiwan;12. Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan;13. Division of Neurosurgery, Department of Surgery, Kaohsiung Armed Forces General Hospital Kaohsiung 802301, Taiwan;14. Center for General Education, Cheng Shiu University, Kaohsiung 833301, Taiwan;15. Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404394, Taiwan;p. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807378, Taiwan;q. Pharmaceutical Biotechnology, Fu Jen Catholic University, New Taipei City 242062, Taiwan |
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| Abstract: | As part of the study of the pathogenesis of oil-induced arthritis in DA rats, the tissue dissemination of arthritogenic oil labelled with 14C has been determined. Rats received labelled hexadecane in arthritogenic doses by the intradermal route and were killed at I and 6 h, 2, 10, 14, 18 and 27 days after injection. Whole-body autoradiography was performed and localization of radioactivity in different organs was investigated. With the exception of the injection site, the lymph nodes showed the highest content of radioactivity throughout the study. Radioactivity could be seen in the popliteal, inguinal and axillary lymph nodes. Detailed examination of lymph nodes revealed at I h radioactivity in the subcapsular sinus of the lymph node. By 10 days the activity had spread to the cortex and paracortex, apart from the subcapsular sinus. The activity in other lymphoid organs, such as the bone marrow and spleen, was more transient, with a peak at 2 days. Oil disseminating to joints was not prominent: very little radioactivity was observed in the knee joints of both non-arthritic and arthritic animals. From these data it is suggested that the adjuvant oil exerts its major proarthritogenic activity in the lymph nodes rather than directly in the joints. |
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