大鼠尾核内一氧化氮对apelin受体mRNA表达的影响

目的研究大鼠尾核内一氧化氮（nitric oxide,NO）的作用机理及其与apelin受体mRNA表达的相关性。方法大鼠尾核内微量注射NO前体L-精氨酸（L-Arg）、N^G-硝基-L-精氨酸甲酯（N^G-nitro-L-arginine methyl ester,L-NAME）和生理盐水（NS）,应用逆转录-聚合酶链反应（RT-PCR）检测大鼠尾核给药4、8、12、24及48h后一氧化氮合酶（neuronal nitric oxide synthase,nNOS）mRNA和apelin受体mRNA表达的变化及二者的相关性。结果注射L-Arg后大鼠尾核nNOS和apelin受体的mRNA表达明显升高,注射L-NAME后大鼠尾核nNOS和apelin受体mRNA表达明显降低,且均有统计学意义。注射L-Arg和L-NAME后,nNOSmRNA和apelin受体mRNA的表达呈正相关。结论在中枢神经系统,尤其是在尾核中,NOS的活性是NO作为神经递质或调质参与包括中枢痛觉调制作用在内的多种生物学作用的关键因素之一,尾核内NO的神经生物学作用可能与apelin受体作用相关。

Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus

Objective To investigate the effect of nitric oxide (NO) on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat. Methods L-Arginine (L-Arg), NG-nitro-L-arginine methyl ester (L-NAME)and normal saline (NS) was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase (nNOS) mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined. Results The expressions of nNOS mRNA and apelin receptor mRNA were both significantly increased after microinjection of L-Arg, but significantly decreased after microinjection of L-NAME compared with the NS control group. The nNOS mRNA had a positive correlation with the expression of apelin receptor mRNA after microinjection of L-Arg and L-NAME. Conclusion The activity of NOS in the central nervous system, especially in the caudate nucleus, is one of the key factors for NO to exert many kinds of biological actions, such as modulation of central pain, as a neurotransmitter. The neurobiological action of NO in rat caudate nucleus may be associated with apelin receptors.