脑室内神经干细胞移植修复谷氨酸导致的成年小鼠神经损伤

目的探讨脑室内神经干细胞移植修复成年小鼠谷氨酸神经毒性损伤的可能性。方法从15日小鼠胚胎脑组织分离神经干细胞,采用免疫细胞化学技术检测细胞Nestin抗原表达;通过免疫荧光染色观察所移植神经干细胞在体内的存活及定位。除对照组外,所有小鼠均以谷氨酸单钠（每天4.0 g/kg）灌胃,连续10天。灌胃后第1 天和10 天,谷氨酸加神经干细胞移植组行神经干细胞脑室内移植（1× 105细胞 /鼠）,对照组和谷氨酸组注射DMEM 液。末次移植后第 11天进行Y迷宫试验,试验结束后进行小鼠脑病理检查,以分析谷氨酸引起的脑功能和形态学上的改变。结果所分离细胞呈Nestin阳性表达;移植10天后所移植神经干细胞在小鼠脑中呈区域特异性存活;脑室内移植神经干细胞能明显促进成年小鼠脑谷氨酸兴奋性毒性损伤的修复。结论脑室内移植神经干细胞可用于疾病或损伤脑组织的修复。

Repair of glutamate-induced excitotoxic neuronal damage mediated by intracerebroventricular transplantation of neural stem cells in adult mice

OBJECTIVE: To investigate a possibility of repairing damaged brain by intracerebroventricular transplantation of neural stem cells (NSCs) in the adult mice subjected to glutamate-induced excitotoxic injury. METHODS: Mouse NSCs were isolated from the brains of embryos at 15-day postcoitum (dpc). The expression of nestin, a special antigen for NSC, was detected by immunocytochemistry. Immunofluorescence staining was carried out to observe the survival and location of transplanted NSCs. The animals in the MSG + NSCs group received intracerebroventricular transplantation of NSCs (approximately 1.0 x 10(5) cells) separately on day 1 and day 10 after 10-d MSG exposure (4.0 g/kg per day). The mice in control and MSG groups received intracerebroventricular injection of Dulbecco's minimum essential medium (DMEM) instead of NSCs. On day 11 after the last NSC transplantation, the test of Y-maze discrimination learning was performed, and then the histopathology of the animal brains was studied to analyze the MSG-induced functional and morphological changes of brain and the effects of intracerebroventricular transplantation of NSCs on the brain repair. RESULTS: The isolated cells were Nestin-positive. The grafted NSCs in the host brain were region-specifically survived at 10-d post-transplantation. Intracerebroventricular transplantation of NSCs obviously facilitated the brain recovery from glutamate-induced behavioral disturbances and histopathological impairs in adult mice. CONCLUSION: Intracerebroventricular transplantation of NSCs may be feasible in repairing diseased or damaged brain tissue.