|
|||||
|
|
| 糖皮质激素对糖尿病大鼠视网膜病变的影响 | |
| 引用本文： | 张博,王玉波,刘文强,刘学政.糖皮质激素对糖尿病大鼠视网膜病变的影响[J].国际眼科杂志,2015,15(8):1319-1321. |
| 作者姓名： | 张博 王玉波 刘文强 刘学政 |
| 作者单位： | 辽宁医学院基础学院解剖学教研室, 中国辽宁省锦州市,121001 |
| 摘 要： | 目的：探讨糖皮质激素对糖尿病( diabetes mellitu,DM)大鼠视网膜神经组织的损伤作用及机制。
方法：利用链脲佐菌素诱导大鼠DM模型,玻璃体内注射腺病毒( DM组)或腺病毒包装的糖皮质激素受体反义寡核苷酸( siGR组)、阴性核苷酸序列( scRNA组)。另取正常SD 大鼠,玻璃体腔注射腺病毒为对照组( CON 组)。12wk后,HE染色检测视网膜神经节细胞( retinal ganglion cell,RGC)密度,测量大鼠视网膜厚度,免疫组织化学和Western-blot检测ROCK表达变化。 结果：与CON组相比,DM组、siGR组及scRNA组糖皮质激素浓度均明显升高(均 P<0.01)。 HE 染色显示,与CON组相比,DM组及scRNA组RGC密度明显降低,视网膜厚度下降,ROCK的蛋白表达增加(均P<0.01),而siGR组无明显变化(均P>0.05)。 结论：抑制糖皮质激素能下调糖尿病大鼠视网膜ROCK表达,恢复视网膜RGC密度降低及视网膜厚度。 |
| 关 键 词： | 糖尿病视网膜病变 视网膜神经节细胞 糖皮质激素 ROCK |
| 收稿时间： | 2015/3/20 0:00:00 |
| 修稿时间： | 2015/7/15 0:00:00 |
Effects of glucocorticoids on retina in diabetic rats |
|
| Bo Zhang,Yu-Bo Wang,Wen-Qiang Liu and Xue-Zheng Liu.Effects of glucocorticoids on retina in diabetic rats[J].International Journal of Ophthalmology,2015,15(8):1319-1321. | |
| Authors: | Bo Zhang Yu-Bo Wang Wen-Qiang Liu and Xue-Zheng Liu |
| Institution: | Department of Anatomy, Foundation Institute of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;Department of Anatomy, Foundation Institute of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;Department of Anatomy, Foundation Institute of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China;Department of Anatomy, Foundation Institute of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China |
| Abstract: | AIM: To investigate the effectand mechanism of glucocorticoids on retinal nerve tissue in diabetes mellitu(DM)rats. METHODS: The DM model was established by a single injection of streptozotocin. Diabetic rats were intravitreal injected with adenovirus vehicle(DM group), or carried glucocorticoids receptor antisense(siGR group), or scrambled nucleotide(scRNA group). Control group(CON group)were normal SD rats intravitreal injected with adenovirus vehicle. Twelve weeks later, the density of retinal ganglion cell(RGC)and retinal thickness were detected by HE staining, and the expression of ROCK was observed by immunohistochemical staining and Western-blot. RESULTS: Compared with CON group, glucocorticoid concentrations were significantly increased in DM group, siGR group and scRNA group(P<0.01, respectively). DM group and scRNA group showed reduction of RGC density and retinal thickness, and up-regulation of ROCK(P<0.01, respectively). While no alterations was detected between CON group and siGR group(P>0.05, respectively). CONCLUSION: Inhibition of glucocorticoids in diabetes reverses the expression of retinal ROCK, RGC density, and retinal thickness. |
| Keywords: | diabetic retinopathy retinal ganglion cells glucocorticoids ROCK |
| 点击此处可从《国际眼科杂志》浏览原始摘要信息 | |
| 点击此处可从《国际眼科杂志》下载免费的PDF全文 | |