低压缺氧对大鼠肾脏功能和结构的影响

目的 观察急性低压缺氧对大鼠肾脏的损害。方法 将３６只雄性Ｗｉｓｔａｒ大鼠随机平均分为Ａ、Ｂ、Ｃ三级。Ａ组为对照组，将Ｂ、Ｃ且置低压舱内上升至５０００ｍ，停留３０ｍｉｎ，予以缺氧。下降后Ｂ组２０ｍｉｎ后，Ｃ组２４ｈ后取静脉血及肾组织，用全自动生化分析仪测定血清尿素氮、肌酐、碱怀磷酸酶、氯离子，并用放免法测定肾组织内皮素。切取肾皮质做光镜及电镜下的病理检查。结果 实验组血清尿素氮和肌酐明显增多（Ｐ〈

Effect of Acute Hypobaric Hypoxia on Renal Function and Structure in Rats

Objective To observe renal damage due to acute hypobaric hypoxia. Method Thirty six male wistar rats were randomly divided into three groups A, B and C(n=12 for each).Group A served as control,while groups B and C were exposed to 5000 m altitude for 30 min.Sample of serum and renal tissues were taken from group B rats 20 min and from group C rats 24 h after the exposure respectively. Result Serum ureanitrogen (BUN) and creatinine (Crea) increased significantly in group B and C rats after exposure to hypoxia(P<0.01).Cl - and alkaline phosphatase (AKP) in serum also increased (P<0.05),but renal endothelin(ET) decreased significantly (P<0.01).All changes in group C rats tended to recover to normal level, except Cl - which continued to increase. Dilation of renal vessels was found under optic microscope,and pathological changes were found in mitochondra and epithelial cells. Conclusion Acute hypobaric hypoxia might be harmful to renal function and structure.