| STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响 |
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| 引用本文： | 陈佳,徐华,张蕴莉.STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响[J].肿瘤防治研究,2010,37(3):263-265. |
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| 作者姓名： | 陈佳 徐华 张蕴莉 |
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| 作者单位： | 1.124010 辽宁盘锦，辽河油田中心医院干诊科,2.检验科；3.辽宁医学院附属 第一医院检验科 |
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| 摘 要： | 目的探讨STAT3反义寡核苷酸的抗肿瘤机制，为乳腺癌的基因治疗提供新的实验依据。方法应用阳离子脂质体介导STAT3反义寡核苷酸转染乳腺癌MCF-7细胞，在倒置显微镜下观察细胞生长情况及形态变化；采用MTT比色法检测细胞增殖状态；流式细胞仪检测细胞凋亡率及细胞周期；Western blot检测STAT3总蛋白和p-STAT3蛋白的表达。结果STAT3反义核苷酸转染乳腺癌细胞后，能抑制MCF-7细胞的增殖，促进MCF-7细胞的凋亡，STAT3蛋白的表达和磷酸化水平下降，出现G1期阻滞。结论STAT3反义核苷酸能抑制MCF-7细胞的增殖，促进MCF-7细胞的凋亡，这将为乳腺癌的治疗提供新的方向。
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| 关 键 词： | STAT3 乳腺癌 反义寡核苷酸 |
| 收稿时间： | 2008-10-29 |
| 修稿时间： | 2008-12-18 |
Effects of STAT3 Antisense Oligonucleotide on Proliferation and Apoptosis of MCF-7 Breast Cancer Cells |
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| CHEN Jia,XU Hua,ZHANG Yun-li.Effects of STAT3 Antisense Oligonucleotide on Proliferation and Apoptosis of MCF-7 Breast Cancer Cells[J].Cancer Research on Prevention and Treatment,2010,37(3):263-265. |
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| Authors: | CHEN Jia XU Hua ZHANG Yun-li |
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| Institution: | 1.Ward of Care; Liaohe Oil Field Central Hospital，Panjin 124010,China,2.Department of Clinical Laboratory; 3.Department of Clinical Laboratory; First Affiliated Hospital of Liaoning Medical College |
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| Abstract: | Objective To observe the influences of STAT3 antisense oligonucleotide on proliferation and apoptosis of MCF-7 breast cancer cells.To study the effects of STAT3 antisense oligonucleotides on MCF-7 breast cancer cells in ovder to provide a new experiment evidence for breast cancer biological therapy.Methods The MCF-7 breast canner cell was transfected with STAT3 antisense oligonucleotide mediated by catholyte liposome.The cells growth and morphology were observed with a convert microscope.The proliferation o... |
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| Keywords: | STAT3 STAT3 Breast cancer Antisense oligonucleotides |
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