雌激素干预的EPCs移植对糖尿病缺血性脑卒中大鼠的治疗作用

目的 探讨雌激素干预的EPCs移植对糖尿病缺血性脑卒中的治疗作用。方法 制备大鼠糖尿病缺血性脑卒中模型，24 h后通过尾静脉移植经PKH26标记的糖尿病EPCs和雌激素干预的糖尿病EPCs。移植3 d后测定各组大鼠脑缺血体积、大鼠行为学变化、缺血部位血管新生及EPCs的归巢情况。结果 与糖尿病脑缺血大鼠相比，雌激素干预的糖尿病EPCs移植能降低脑缺血体积、改善行为学评分和缺血部位的血管新生及促进EPCs归巢到损伤部位(P<0.05)。结论 雌激素干预的糖尿病EPCs移植，通过促进EPCs归巢和血管新生对糖尿病缺血性脑卒中有较好的治疗作用。

Therapeutic effects of estrogen-intervened EPCs transplantation on diabetic ischemic stroke rats

Objective To explore the therapeutic effects of estrogen-intervened endothelial progenitor cells( EPCs) transplantation on diabetic ischemic stroke rats. Methods PKH26-labeled diabetic EPCs and estrogen-intervened diabetic EPCs were injected into rats via the tail vein 24 h after cerebral ischemia. Cerebral ischemic volume, behavioral changes, ischemic site vascularization and homing of EPCs were measured 3 d after EPCs injection. Results Compared with diabetic ischemic rats, estrogen-intervened EPCs transplantation had reduced infarct volumes, improved behavioral scores and ischemic site revascularization and promoted homing of EPCs to sites of injury(P<0.05). Conclusion Estrogen-intervened EPCs transplantation had a better therapeutic effect on diabetic ischemic stroke by promoting EPCs homing to injury site and EPCs-medicated neovascularization .