| Semaphorin 7A及其受体在A549细胞转移中的作用 |
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| 引用本文： | 闫雪,崔蕊,熊晓毅,张敏龙. Semaphorin 7A及其受体在A549细胞转移中的作用[J]. 西部医学, 2023, 35(1): 72-76 |
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| 作者姓名： | 闫雪 崔蕊 熊晓毅 张敏龙 |
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| 作者单位： | 咸阳职业技术学院医学院；空军军医大学预防医学系;空军军医大学唐都医院呼吸科；解放军总医院呼吸与危重症学部 |
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| 基金项目： | 咸阳职业技术学院重大科学研究项目（2019KYA02） |
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| 摘 要： | 目的 观察SEMA7A及其受体在A549细胞转移中的作用机制。方法 利用小干扰RNA技术检测SEMA7A及其受体Plexin C1相互作用对A549细胞转移及其相关信号通路的影响。结果 SEMA7A及其受体Plexin C1在A549细胞中表达明显高于正常肺泡上皮细胞；SEMA7A增加了肿瘤细胞转移关键因子CCR-7、MMP-2及MMP-9的表达，但当SEMA7A及其受体Plexin C1沉默表达后，CCR-7、MMP-2及MMP-9的表达出现下降，同时A549细胞转移受到明显抑制。结论 SEMA7A及其受体Plexin C1相互作用促进了A549细胞的转移。
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| 关 键 词： | Semaphorin 7A Plexin C1 A549细胞 转移 |
The role of Semaphorin 7A and its receptor in the migration of A549 cells |
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| YAN Xue,CUI Rui,XIONG Xiaoyi,ZHANG Minlong. The role of Semaphorin 7A and its receptor in the migration of A549 cells[J]. , 2023, 35(1): 72-76 |
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| Authors: | YAN Xue CUI Rui XIONG Xiaoyi ZHANG Minlong |
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| Affiliation: | School of Medicine, Xianyang Vocational Technical College, Xianyang 712000, Shanxi, China; Department of Preventive Medicine, Air force Medical University, Xi''an 710032, China;; Department of Respiration, Tangdu Hospital, Air force Medical University, Xi''an 710038, China; Department of Respiratory and Critical Care Medicine, PLA General Hospital, Beijing 100091, China |
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| Abstract: | To observe the mechanism of interaction between SEMA7A and its receptor in the migration of A549 cells. Methods The effect of interaction between SEMA7A and plexin C1 in A549 cells migration and migration related signaling pathway were detected using SEMA7A and plexin C1 small interfering RNA. Results The expression of SEMA7A and plexin C1 were significantly increased in A549 cells compared with normal alveolar epithelial cells. SEMA7A increased the expression of tumor migration related protein CCR-7, MMP-2 and MMP-9. However, the expression of CCR-7, MMP-2 and MMP-9 decreased when SEMA7A and plexin C1 were silenced. Conclusion The interaction between SEMA7A and plexin C1 promoted the migration of A549 cells. |
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| Keywords: | Semaphorin 7A Plexin C1 A549 cells Migration |
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