藜芦有效成分提取工艺优化

[目的]借助正交实验与统计分析软件（JMP）实验设计模型优化藜芦有效成分的提取工艺。[方法]采用单因素实验、正交实验优化乙醇体积分数、液料比、提取时间、提取次数，通过分析芥芬胺和藜芦胺的含量差异确定提取工艺。[结果]模型所得最佳工艺参数与正交实验结果相符，为乙醇体积分数70%，时间2 h，提取3次，液料比15∶1（mL/g）。在此工艺下，芥芬胺和藜芦胺含量分别为1.60、2.98 mg/g，相对标准偏差（RSD）分别为1.0%、1.1%。[结论]该提取工艺稳定，可用于藜芦中有效成分的提取。

Optimization of extraction process of effective ingredients in Veratrum Nigrum L.

[Objective] With the help of the experimental design model and orthogonal experiment of JMP software,the extraction process of the active components of Veratrum Nigrum L. was optimized.[Methods] Single factor experiment and orthogonal experiment were used to optimize the ethanol volume fraction,material-to-liquid ratio,extraction time,and extraction times. The extraction process was determined by analyzing the content difference of jervine and veratramine.[Results] Optimal process obtained by the model is equal to the actual experimental results. The best process parameters as follows:the volume fraction of ethanol was 70%,2 hours each time,the extraction times were 3 times and ratio of liquid to material was 15:1 (mL/g). Under the optimized conditions,the contents of jervine and veratramine were 1.60 mg/g and 2.98 mg/g,and the RSD was 1.0% and 1.1%,respectively.[Conclusion] The extraction process was stable and could be used for the extraction of effective ingredients in Veratrum Nigrum L.