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50种中药甲醇提取物对嗜水气单胞菌的体外抑菌及抗生物膜活性的评价
引用本文:傅钰瑛,陈小萌,Srinivasan Ramanathan,李珍,刘柱,林向民.50种中药甲醇提取物对嗜水气单胞菌的体外抑菌及抗生物膜活性的评价[J].现代药物与临床,2023,46(1):64-71.
作者姓名:傅钰瑛  陈小萌  Srinivasan Ramanathan  李珍  刘柱  林向民
作者单位:福建船政交通职业学院 安全与环境学院, 福建 福州 350007;福建农林大学 生命科学学院, 福建省农业生态过程与安全监控重点实验室, 福建 福州 350002;漳州卫生职业技术学院, 福建 漳州 363000;海南大学 生命与药物学院 分子生物实验室, 海南 海口 570228
基金项目:国家自然科学基金资助项目(31670129);中国博士后科学基金面上资助项目(2019M662214);博士科研启动专项基金(20220105)
摘    要:目的 评价50种中药甲醇提取物对嗜水气单胞菌的体外抑菌效果以及抗生物膜生成活性。方法 采取甲醇浸提的方法制备中药甲醇提取物,计算提取率;利用多功能酶标仪检测600nm处的吸光度(A600)值测定各提取物(0.1、0.2、0.4、0.8、1.6、3.2mg·mL-1)作用16h对嗜水气单胞菌的抑菌率;结晶紫染色法结合多功能酶标仪测定各提取物的抗生物膜活性。结果 苏木、白芍、侧柏叶等32种中药甲醇提取物对嗜水气单胞菌抑菌效果明显,其中苏木甲醇提取物的抑菌效果最显著,最大抑菌率达95.56%,起效质量浓度为0.1mg·mL-1;石菖蒲、大青叶、广藿香等20种中药甲醇提取物明显抑制嗜水气单胞菌生物被膜的生成,其中白芍的最大抑制率达96.78%。结论 夏枯草、野菊花、大青叶、赤芍、石菖蒲、地肤子、连翘、苏木、肉桂、女贞子、白芍、甘草、肉豆蔻、鸡血藤、乌药、莪术、侧柏叶17种中药对嗜水气单胞菌同时具有抑菌和抑制生物膜生长活性。

关 键 词:中药  嗜水气单胞菌  体外抑菌  抗菌活性  抗生物膜活性  苏木  白芍
收稿时间:2022/7/27 0:00:00

In vitro antibacterial and anti-biofilm activities of 50 kinds of traditional Chinese medicine methanol extracts against Aeromonas hydrophila
FU Yuying,CHEN Xiaomeng,Srinivasan Ramanathan,LI Zhen,LIU Zhu,LIN Xiangmin.In vitro antibacterial and anti-biofilm activities of 50 kinds of traditional Chinese medicine methanol extracts against Aeromonas hydrophila[J].Drugs & Clinic,2023,46(1):64-71.
Authors:FU Yuying  CHEN Xiaomeng  Srinivasan Ramanathan  LI Zhen  LIU Zhu  LIN Xiangmin
Institution:School of Safety and Environment, Fujian Chuanzheng Communications College, Fuzhou 350007, China;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Zhangzhou Health Vocational and Technical College, Zhangzhou 363000, China;Laboratory of Molecular Microbiology, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, China
Abstract:Objective To evaluate the in vitro antibacterial and anti-biofilm activities of 50 kinds of tranditional Chiese medicine (TCM) extracts against Aeromonas hydrophila. Methods The methanol extract was prepared by methanol extraction and the extraction rate was calculated. Multifunctional enzyme marker was used to detect the absorbance (A600 nm) value at 600 nm to determine the inhibitory rate of each extract (0.1, 0.2, 0.4, 0.8, 1.6, 3.2 mg·mL-1) against A.hydrophila for 16 h. The antibiofilm activity of the extracts was determined by crystal violet staining and multifunctional enzyme marker. Results Totally 32 kinds of TCM methanol extracts, including Caesalpinia sappan, Paeonia iactiflora and Platycladus orientalis and so on, showed obvious bacteriostatic effect on A.hydrophila, among which Sappan Lignum showed the most significant bacteriostatic effect with the maximum bacteriostatic rate of 95.56% and effective concentration of 0.1 mg·mL-1. The methanol extracts of 20 kinds of TCM, such as Acorus tatarinowii, Isatis indigotica and Pogostemon cablin and so on, inhibited the biofilm formation of A.hydropaeoniae, and the maximum inhibitory rate of Paeonia iactiflora was 96.78%. Conclusion 17 TCM have bothe antibacterial and anti-biofilm activity, including Prunella vulgaris, Chrysanthemum indicum, Isatis indigotica, Paeonia iactiflora, Acorus tatarinowii, Kochia scoparia, Forsythia suspensa, Caesalpinia sappan, Cinnamomum cassia, Ligustrum lucidum, Paeonia iactiflora, Glycyrrhiza uralensis, Myristica fragrans, Spatholobus suberectus, Lindera aggregata, Curcuma phaeocaulis, Platycladus orientalis.
Keywords:traditional Chinese medicine  Aeromonas hydrophila  antibacterial in vitro  antimicrobial activity  anti-biofilm activity  Caesalpinia sappan  Paeonia iactiflora
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