金钗石斛对老龄小鼠抗氧化作用的实验研究

目的了解金钗石斛对老龄小鼠的抗氧化作用。方法将40只雄性老龄小鼠随机分为4组:即空白对照组、低剂量组、中剂量组和高剂量组。低、中、高剂量组分别经口灌胃给予0.06、0.12和0.36g/kg.bw金钗石斛溶液,空白对照组给予同体积的蒸馏水。持续给药30d后,测定各组小鼠血中丙二醛(MDA)含量、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活力。结果高剂量组小鼠血中MDA水平下降,与对照组相比,差异有统计学意义(P0.05),SOD活力和GSH-Px活力水平升高,且与对照组相比,差异均有统计学意义(P0.05)。结论金钗石斛对老龄小鼠具有抗氧化作用。

Experimental Study on Antioxidant Function of Dendrobium Nobile Lindl in Aged Mice

Objective To study the antioxidant effect of dendrobium nobile lindl in aged mice.Methods Forty aged male mice were randomly divided into four groups （each n=10）.The animals in control group were orally administered with distilled water,and the animals in three treatment groups were orally administered with dendrobium nobile lindl at the dosages of 0.06 g/kg·bw,0.12 g/kg·bw and 0.36 g/kg·bw respectively once daily for 30 days.The serum concentration of malondialdehyde （MDA） and the activities of superoxide dismutase （SOD） and glutathione peroxidese （GSH-Px） were detected by ultraviolet-visible spectrophotometry.Results The serum concentration of MDA in the mice treated with 0.36 g/kg·bw dendrobium nobile lindl were significantly lower than that in control group,and the difference was statistically significant （P〈0.05）.The serum levels of SOD and GSH-Px in the mice treated with 0.36 g/kg·bw dendrobium nobile lindl were significantly higher than those in control group,and the difference was statistically significant （P〈0.05）.Conclusions Dendrobium nobile lindl has antioxidant effects in aged mice.