Preserved neurotransmitter receptor binding following ischemia in preconditioned gerbil brain |
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Authors: | Hiroyuki Kato Tsutomu Araki Kyuya Kogure |
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Institution: | Department of Neurology, Tohoku University School of Medicine, Sendai, Japan. |
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Abstract: | Preconditioning the brain with sublethal ischemia induces tolerance to subsequent ischemic insult. Using 3H]quinuclidinyl benzilate (QNB), 3H]MK 801, 3H]cyclohexyladenosine, 3H]muscimol, and 3H]PN200-110, we investigated the alterations in neurotransmitter receptor and calcium channel binding in the gerbil hippocampus following ischemia with or without preconditioning. Two-minute forebrain ischemia, which produced no neuronal damage, resulted in no alterations in binding except for a slight reduction in 3H]QNB binding in the CA1 subfield. Three-minute ischemia destroyed the majority of CA1 pyramidal cells and caused, in CA1, reductions in binding of all ligands used. Preconditioning with 2-min ischemia followed by 4 days of reperfusion protected against CA1 neuronal damage and prevented the reductions in binding although 3H]QNB and 3H]PN200-110 binding transiently decreased in the early reperfusion period, suggesting down-regulation. Thus, preconditioning protects against damage to the neurotransmission system as well as histopathological neuronal death. |
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Keywords: | Cerebral ischemia Preconditioning Tolerance Neurotransmitter systems Receptor Autoradiography Hippocampus Gerbil |
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