反相高效液相色谱法测定氨氯缬沙噻嗪片中3种组分的溶出度

目的:建立可同时测定氨氯缬沙噻嗪片3种组分溶出度的反相高效液相色谱方法。方法:采用Inertsil ODS-3 C18色谱柱(250 mm×4.6 mm,5μm),以20 mmol.L-1磷酸二氢钾溶液(磷酸调节pH值至3.5)-乙腈(55∶45)为流动相,流速1.0 mL.min-1,检测波长225 nm。结果:氨氯地平、缬沙坦和氢氯噻嗪的线性范围分别为1.3～20.1μg.mL-1(r=0.999 9),39.8～636.8μg.mL-1(r=0.999 9)和3.1～49.4μg.mL-1(r=1.000 0);回收率分别为100.5%,100.6%和100.4%。结论:方法简便快速、重复性好,可用于同时测定氨氯缬沙噻嗪片3种组分的溶出度。

Simultaneous HPLC determination of the dissolution of three components in compound amlodipine-valsartan-hydrochlorothiazide tablets

Objective: To develop an HPLC method for simultaneous determination of dissolution of amlodipine-valsartan-hydrochlorothiazide tablets.Methods: The method was achieved on an Inertsil ODS-3 C18 column(250 mm×4.6 mm,5 μm),using a mixture of potassium dihydrogen phosphate solution(adjust to pH 3.5 with H3PO4)-acetonitrile(55∶45) as the mobile phase.The flow rate was 1.0 mL·min-1 and UV detection was at 225 nm.Results: The calibration curves of amlodipine,valsartan and hydrochlorothiazide were linear in the range of 1.3~20.1 μg·mL-1(r=0.999 9),39.8~636.8 μg·mL-1(r=0.999 9) and 3.1~49.4 μg·mL-1(r=1.000 0),respectively.The average recoveries were 100.5%,100.6% and 100.4%,respectively.Conclusion: The established method is simple,rapid and reproducible for simultaneous determination of dissolution of amlodipine-valsartan-hydrochlorothiazide tablets.