| 关节内骨折后受损软骨细胞凋亡的实验研究 |
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| 引用本文： | 司全明,侯筱魁,唐敏.关节内骨折后受损软骨细胞凋亡的实验研究[J].中国矫形外科杂志,2002,9(7):675-677. |
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| 作者姓名： | 司全明 侯筱魁 唐敏 |
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| 作者单位： | 1. 宁波市第一医院骨科,宁波市骨科研究所,宁波市,315010
2. 上海第二医科大学附属第九人民医院骨科 |
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| 基金项目： | 上海市科技发展基金项目 ,项目编号 :0 0 4 1 1 90 2 1 |
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| 摘 要： | 目的：探讨关节内骨折后受损软骨的变化。方法：利用冲击在兔膝关节内造成骨折，利用TUNEL、流式细胞计数，透射电镜、DAPI染色等方法对受损软骨进行动态观察。结果：关节内骨折后2周，受损软骨细胞发生坏死及凋亡。1月后受损软骨细胞仍进行性凋亡。3－6月后，损伤软骨周围的细胞也发生凋亡，损伤部软骨细胞增生，其中部分增生细胞凋亡。结论：关节内骨折后软骨细胞进行性凋亡，可能是创伤后骨关节炎发生的原因之一。
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| 关 键 词： | 关节内骨折 受损软骨 细胞凋亡 实验研究 动物模型 |
| 文章编号： | 1005-8478(2002)07-0675-03 |
| 修稿时间： | 2001年6月26日 |
Injured Chondrocytes Apoptosis following Intra-articular Fracture |
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| SI Quan ming,HOU Xiao kui,TANG Min.Injured Chondrocytes Apoptosis following Intra-articular Fracture[J].The Orthopedic Journal of China,2002,9(7):675-677. |
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| Authors: | SI Quan ming HOU Xiao kui TANG Min |
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| Institution: | SI Quan ming,HOU Xiao kui,TANG Min.Department of Orthopaedics,Ningbo the First Hospital,Ningbo 315010 |
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| Abstract: | Objective: To explore the changes of injured cartilage after intra articular fracture.Methods:Intra articular fracture was induced by an impact in rabbit knees.TUNEL,fluorecence activated cell sorter(FACS),transmission electron microscopy (TEM),DAPT staining were applied to study the injured cartilage obtained at different intervals after fracture.Results:Within 2 weeks,injured chondrocytes died and apoptosis.After 1 month,chondrocyte apoptosis in the injured cartilage continued.3 to 6 months following fracture,apoptotic chondrocytes were found in proliferative chondrocytes and in the cartilage around injured cartilage.Conclusions:Chondrocyte apoptosis may be one of the pathways for osteoarthritis after intra articular fracture. |
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| Keywords: | Cartilage Injury Apoptosis Osteoarthritis |
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