亚甲基蓝／光化学法灭活血浆中指示病毒及对血浆？…

目的 探讨亚甲基蓝光化学法灭活血浆中模型病毒的效果。方法 用ＶＳＶ－Ｉｎｄｉａｎａ株作为指示病毒。接种于Ｖｅｒｏ单层细胞中，孵育３９℃２４ｈ用微量细胞病变法，进行病毒滴定。并用小量试验观察病毒灭活效果及对血浆蛋白的影响。结果 亚甲基蓝（ｍｅｔｈｙｌｅｎｅ ｂｌｕｅ ＭＢ）结合可见光照射可有效灭活血浆中的指示病毒，极低浓度的染料加光照可完全灭活血浆中大于６ｌｏｇ１０ＴＣＩＤ５０的水泡性口膜炎病毒（Ｖ

Photodynamicinactivation of model virus in human plasma by methylene blue and its influence on plasmaproteins

OBJECTIVE: To determine whether photochemical method can inactivate viruses in human plasma efficiently, and to what extent it affects the protein in human plasma. METHODS: Vascular stomatitis virus (VSV) Indiana strain was used as the indicating virus. It was inoculated on Vero cell monolayer, incubated in 37 degrees C for 24 hours, microtitered by cytopathogenic effect. Small amount experiments were carried out to observe virus inactivation and the influence on plasma proteins. RESULTS: Methylene blue (MB) in combination with visible light can inactivate the model virus in human plasma efficiently. The dye in very low concentration combined with light was sufficient to achieve a reduction of VSV titer of more than 6 log10 TCID50. Compared with the untreated plasma, neither the activities of blood clotting factors nor the immunological properties of the plasma proteins and the blood albumin were reduced significantly. In this experiment, the dose of MB used for photoinactivation of the virus was very low, about a hundred fold lower than that of methemoglobinemia used in clinical treatment. Furthermore, heat of the light and photochemical reaction are two import factors for decreasing of clotting factor's activity. CONCLUSIONS: The results of this preliminary study suggest the potential application of MB-photodynamic inactivation of plasma virus is an useful approach in clinical practice.