| 叶酸缺乏对HBL-100和MCF-7细胞凋亡的影响 |
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| 引用本文： | 欧敬民,张一楚,费哲为,张纪伟,董谦,姜宏华,杜鹏. 叶酸缺乏对HBL-100和MCF-7细胞凋亡的影响[J]. 肠外与肠内营养, 2008, 15(1): 26-28 |
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| 作者姓名： | 欧敬民 张一楚 费哲为 张纪伟 董谦 姜宏华 杜鹏 |
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| 作者单位： | 上海第二医科大学附属新华医院普通外科,上海,200092 |
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| 摘 要： | 目的:测定叶酸缺乏对人乳腺细胞HBL-100和乳腺癌细胞MCF-7凋亡的影响.方法:将HBL-100和MCF-7细胞在叶酸缺乏的培养液(实验组)和正常培养液(对照组)中培养30d,用电镜和流式细胞仪测定叶酸缺乏对HBL-100和MCF-7细胞凋亡的影响.结果:HBL-100细胞在两种培养液中生长30d后,观察结果显示叶酸缺乏可引起HBL-100细胞凋亡增高,而对MCF-7细胞凋亡率则无影响.结论:叶酸缺乏可增加HBL-100细胞的凋亡率.
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| 关 键 词： | 乳腺癌 叶酸缺乏 细胞培养 细胞凋亡 |
| 文章编号： | 1007-810X(2008)01-0026-03 |
| 修稿时间： | 2007-09-20 |
The effect of folacin deficiency on the apoptosis of HBL-100 and MCF-7 |
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| OU Jing-min,ZHANG Yi-chu,FEI Zhe-wei,ZHANG Ji-wei,DONG Qian,JIANG Hong-hua,DU Peng. The effect of folacin deficiency on the apoptosis of HBL-100 and MCF-7[J]. Parenteral & Enteral Nutrition, 2008, 15(1): 26-28 |
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| Authors: | OU Jing-min ZHANG Yi-chu FEI Zhe-wei ZHANG Ji-wei DONG Qian JIANG Hong-hua DU Peng |
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| Affiliation: | OU Jing-min,ZHANG Yi-chu,FEI Zhe-wei,ZHANG Ji-wei,DONG Qian,JIANG Hong-hua,DU Peng(Department of General Surgery,Xinhua Hospotal,Shanghai Second Medical University,Shanghai 200092,China) |
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| Abstract: | Objective:To deuermine whether folacin deficiency affects the apoptosis of HBL-100 and MCF-7.Methods:HBL-100 and MCF-7 were cultured in a medium either in the absence(experimental group) or presence(control group) of folacin for 30 days.Apoptosis was evaluated by FCM and electron microscope.Results:In the HBL-100 cell line of experimental group,compared with the control group,after 30 days of culture,apoptosis was significantly higher,whereas MCF-7 not.Conclusion:Folacin deficiency increases the ratio of ap... |
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| Keywords: | Breast cancer Folacin deficiency Cell culture Apoptosis |
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