蹄叶橐吾地上部分乙醇提取物对小鼠急性乙醇中毒的保护作用

目的:探讨蹄叶橐吾地上部分乙醇提取物对小鼠急性酒精中毒的保护作用。方法:采用一次ig给予56度白酒,记录小鼠翻正反射消失(醉酒)至恢复(醒酒)所需时间(min)及采用52度白酒ig复制小鼠急性乙醇中毒模型,并检测其血清丙氨酸氨基转移酶(ALT)活性及丙二醛(MDA)、甘油三脂(TG)含量和肝组织中ALT、谷胱甘肽过氧化物酶(GSH-Px)活性及MDA,TG含量,观察蹄叶橐吾对小鼠急性乙醇中毒的保护作用。结果:与模型组相比,蹄叶橐吾组小鼠出现翻正反射消失(醉酒)的潜伏期明显延长(P0.05),醒酒时间明显缩短(P0.05);蹄叶橐吾组小鼠血清及肝组织中TG含量及ALT活性均明显降低,GSH-Px活性明显增高。结论:蹄叶橐吾对小鼠急性乙醇中毒有一定预防性的保护作用。

Protective Effect of Ligularia fischeri Ledeb Turz. against Acute Alcohol Intoxication in Mice

Objective: To investigate the protective effect of the ethanol extract of Ligularia fischeri Ledeb Turcz. in mice on acute alcohol intoxication. Method: We recorded the duration from disappearance of righting reflex (drunk) to restore (hangover) in mice, after given a gavage of 56 degrees liquor. In addition, alcohol-induced liver injury model of mice was established by acute gavage of 52 degrees liquor, and serum alanine aminotransferase (ALT) activity, malondialdehyde (MDA), triglyceride (TG) content were tested. Activity of glutamic-pyruvic transaminase (ALT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), triglyceride (TG) in liver tissue was also determined. Result: The ethanol extract of L. fischeri significantly prolonged the incubation period of righting reflex disappeared (drunk) (P<0.05), reduced hangover time (P<0.05); significantly depressed TG and ALT levels (P<0.05) in serum and liver tissue; and significantly increased GSH-Px activity (P<0.01) in liver tissue. Conclusion: L. fischeri may have a protective effect on acute alcohol intoxication in mice.