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| 多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用 | |
| 引用本文： | 赵静博,邹文进,付馨余.多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用[J].国际眼科杂志,2013,13(6):1090-1092. |
| 作者姓名： | 赵静博 邹文进 付馨余 |
| 作者单位： | 中国广西壮族自治区南宁市,广西医科大学第一附属医院眼科;中国广西壮族自治区南宁市,广西医科大学第一附属医院眼科;中国广西壮族自治区南宁市,广西医科大学第一附属医院眼科 |
| 基金项目： | 国家自然科学基金(No.81160119); 广西自然科学基金(No.2012GXNSFAA053107); 广西高等学校立项科研项目(No.201204LX064) |
| 摘 要： | 目的:观察多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用。方法:健康SD大鼠32只,随机分为对照组、多西环素组,每组16只大鼠。建立角膜碱烧伤模型后,多西环素组予3g/L多西环素眼液点眼,对照组予眼液溶媒点眼。分别于碱烧伤后第3,7,14,21d观察计算炎症指数,角膜取材后进行病理切片及炎症细胞计数,行ICAM-1的ELISA检测。结果:多西环素组各时间点结膜充血、角膜水肿较对照组轻,炎症指数均明显低于对照组,差异有统计学意义(P<0.05)。多西环素组各时间点炎症细胞计数少于对照组(P<0.05)。多西环素组各时间点大鼠角膜的ICAM-1表达低于对照组(P<0.05)。结论:多西环素可以抑制碱烧伤大鼠角膜的炎症细胞浸润,其机制可能是抑制ICAM-1的表达。 |
| 关 键 词： | 多西环素 碱烧伤 角膜 炎症细胞 |
| 收稿时间： | 2013/1/29 0:00:00 |
| 修稿时间： | 2013/5/27 0:00:00 |
Doxycycline inhibits the infiltration of inflammatory cells in rat alkali burn corneas |
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| Jing-Bo Zhao,Wen-Jin Zou and Xin-Yu Fu.Doxycycline inhibits the infiltration of inflammatory cells in rat alkali burn corneas[J].International Journal of Ophthalmology,2013,13(6):1090-1092. | |
| Authors: | Jing-Bo Zhao Wen-Jin Zou and Xin-Yu Fu |
| Institution: | Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China |
| Abstract: | AIM: To investigate the inhibited effect of Doxycycline on the infiltration of inflammatory cells in rat alkali burn corneas. METHODS: Corneas of 32 SD rats were injured with 1mol/L NaOH, then divided into two groups: control and doxycycline-treated. All agents were administered topically 4 times daily. Slit-lamp microscope was performed and inflammatory index was calculated at 3, 7, 14 and 21 days after injury. Then 4 rats were randomly sacrificed and each cornea was divided into two parts, one for histopathology, the other for ICAM-1 ELISA assay. RESULTS:In control group, the inflammatory index and the number of inflammatory cell was higher than the doxycycline-treated dramatically at all time points(P<0.05). Compared with control group, corneal ICAM-1 expression decreased significantly in doxycycline-treated group at all time points(P < 0.05). CONCLUSION:Doxycycline may inhibit inflammatory cell infiltration by down-regulating ICAM-1 expression. |
| Keywords: | Doxycycline alkali burn cornea inflammatory cells |
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