青蒿琥酯对腺样囊性癌NACC细胞裸鼠移植瘤抑制效果的研究

目的:观察青蒿琥酯(ART)对腺样囊性癌裸鼠移植瘤的治疗效果,并探讨其作用机制。方法:裸鼠背部皮下接种涎腺腺样囊性癌NACC细胞系,建立移植瘤模型,接种7天后始见成瘤时,随机将动物分为5组,即生理盐水对照组、ART 50.0 mg.kg-1组、ART 100.0 mg.kg-1组、ART 200.0 mg.kg-1组、DDP 2.0 mg.kg-1组。每组5只,隔天给药,称裸鼠体重,给药8次后取瘤称重,采用免疫组化法、Western blot法检测Bcl-2,ERK2,VEGF的表达。结果:ART 200.0 mg.kg-1组以及DDP 2.0 mg.kg-1组移植瘤瘤重较生理盐水对照组明显减轻(P<0.05)。ART 200.0 mg.kg-1组与DDP 2.0 mg.kg-1组相比,抑瘤效果无显著性差异(P>0.05)。DDP 2.0 mg.kg-1组体重减轻明显,极度瘦弱,其余各组体重及生活状态无明显改变。瘤体标本免疫组化及Western blot法检测均发现ART 100.0 mg.kg-1可引起细胞凋亡并下调Bcl-2、ERK2以及VEGF的表达,这种下调作用在ART 200.0 mg.kg-1组更为明显,其与DDP 2.0 mg.kg-1组效果相似。结论:青蒿琥酯可有效抑制腺样囊性癌裸鼠移植瘤的生长,且毒副作用小,其作用机制可能与下调肿瘤VEGF,Bcl-2,ERK2的表达相关。

Inhibition Effects of Artesunate on Human Palatal Salivary Gland Cell Line of Adenoid Cystic Carcinoma in Vivo

This study was aimed to investigate treatment effects of artesunate (ART) on a palatal salivary gland cell line of adenoid cystic carcinoma named NACC in vivo. NACC cells were inoculated subcutaneously into BABL-C nude mice. The xenografting mice were divided into the control group, increasing dose of ART treated groups and DDP treated group. Treatments started on the 7th day when solid tumors were palpated. Drugs were given every other day for 8 times. The animals were sacrificed on the 23th day. And then, tumors were excised and weighted. Tumors were also investigated by immunohistochemistry and Western blotting. The results showed that xenograft of athymic nude mouse were significantly inhibited by ART 200 mg/kg and DDP 2.0 mg/kg (P <0.05) in vivo. ART 200 mg·kg-1 and DDP 2.0 mg·kg-1 had the same effects in suppressing the growth of nude mice xenograft. With the increasing of ART concentration, the expression of VEGF, Bcl-2 and ERK2 had been down-regulated in transplanted tumors, especially in ART 100 mg·kg-1, ART 200 mg·kg-1 and DDP 2.0 mg·kg-1 groups (P < 0.05). It was concluded that ART could significantly inhibit the growth of NACC cell athymic mice xenograft. The mechanism might related to tumor growth-inhibiting, apoptosis-inducing and neovascularization-suppressing.