人胎结肠及直肠内胃泌素、血管活性肠肽、胰多肽、生长抑素免疫反应阳性细胞的个体发生

目的：探讨人胎结肠及直肠胃泌素(Gas)、血管活性肠肽(VIP)、胰多肽(PP)和生长抑素(SS)免疫反应阳性(IR)细胞的个体发生及其相互关系。方法：免疫组织化学SABC法及细胞计数。结果：结肠内Gas-、PP-、SS-IR细胞始见于第9w，VIP-IR细胞于第18w出现。直肠内未见Gas-IR细胞，PP-IR细胞始见于第9w，SS-、VIP-IR细胞于第11w出现。结肠内Gas-IR细胞随胎龄增长数量减少，于第26w未见。PP-IR细胞数量在结肠内随胎龄增长先少后多，直肠内未见明显变化。VIP-IR细胞在胎期结肠、直肠少，而SS-IR细胞较多，整个胎期VIP-、SS-IR细胞数量无明显增减。结论：VIP、PP和SS存在于人胎结肠、直肠的内分泌细胞，Gas仅在结肠内分泌细胞中有表达，提示各种内分泌激素对胎儿结肠及直肠的发育起调节作用。

Ontogeny on gastrin, vasoactive intestinal peptide, pancreatic peptide, somatostatin-immunoreactive cells in colon and rectum of human fetus

Objective : To investigate the ontogeny of Gas-, VIP-, PP-, SS-immunoreactive (IR) cells and explore the relationship of them in human fetal colon and rectum. Methods : Immunohistochemical SABC method and cell counting were used in the experiment. Results : Gas-,PP-,SS-IR cells began to present in the fetal colon on the 9th week. VIP-IR cells occurred on the 18th week. In the fetal rectum, Gas-IR cells were not observed. PP-IR cells began to present by the 9th week; SS-, VIP-IR cell occurred on the 11th weeks. With the growth of fetus, in the fetal colon the number of Gas-IR cells declined and no Gas-IR cells was found on the 26th weeks. In the fetal colon with the growth of fetus the number of the PP-IR cells started to descend and ascended subsequently. In the fetal rectum PP-IR cells did not alter obviously. In the fetal colon and rectum VIP-IR cells were less, but SS-IR cells were more. In the whole fetal period, the number of these 2 kinds of cells did not change distinctly. Conclusion : VIP,PP and SS are expressed in endocrine cells of human fetal colon and rectum, and Gas is only expressed in those of human fetal colon and they may play regulative role for the development of fetus colon and rectum.