| 脂肪酸合酶抑制剂——浅蓝菌素阻遏K562细胞增殖及诱导凋亡 … |
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| 引用本文： | 陈宝安,黄培林.脂肪酸合酶抑制剂——浅蓝菌素阻遏K562细胞增殖及诱导凋亡 …[J].中华血液学杂志,2000,21(5):244-246. |
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| 作者姓名： | 陈宝安 黄培林 |
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| 作者单位： | 南京铁道医学院附属医院血液科(陈宝安!210009，戴振声!210009)，南京铁道医学院病理教研室(黄培林)，上海医科大学华山医院血液科(林果为) |
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| 基金项目： | 江苏省科委研究基金! (BJ970 71)，南京铁道医学院研究基金! ( 98 X 4) |
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| 摘 要： | 目的 探讨脂肪酸合酶抑制剂－浅蓝菌素对５６２白血病细胞增殖的影响及机制，并观察浅蓝菌素对人皮肤成纤维细胞增殖的影响。方法 应用ＭＴＴ法观察浅蓝菌素对Ｋ５６２白血病细胞、人皮肤成纤维细胞增殖的抑制率；应用流式细胞术，ＤＮＡ凝胶电泳进行凋亡的检测和观察。
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| 关 键 词： | 脂肪酸合酶抑制剂 浅蓝菌素 白血病 细胞增殖 |
| 修稿时间： | 1999-09-06 |
Proliferation inhibition and apoptosis induction of K562 cells by fatty acid synthase inhibitor--cerulenin] |
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| CHEN Baoan,HUANG Peilin,DAI Zhensheng,et al..Proliferation inhibition and apoptosis induction of K562 cells by fatty acid synthase inhibitor--cerulenin][J].Chinese Journal of Hematology,2000,21(5):244-246. |
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| Authors: | CHEN Baoan HUANG Peilin DAI Zhensheng |
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| Institution: | Department of Hematology, Affiliated Hospital, Nanjing Railway Medical College, Nanjing 210009, China. |
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| Abstract: | OBJECTIVE: To investigate the effect of fatty acid synthase (FAS) inhibitor--cerulenin on K562 leukemia cells and its mechanism. METHODS: Inhibition rate of cerulenin on K562 leukemia cells was assayed by MTT method, cell apoptosis by flow cytometry (FCM) and agarose gel electrophoresis. RESULTS: When treated with 10(-9) - 10(-5) mol/L of cerulenin for 24 h, the proliferation of K562 cells was obviously inhibited with dose related effect. At the same concentrations, the inhibition rates of human skin fibroblasts were all lower than 30%. When K562 cells were treated for 12 h with 50 microg/ml and 60 microg/ml of cerulenin, the apoptosis rate revealed by FCM was 42.30% and 38.8%, respectively, and DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis. CONCLUSION: Fatty acid synthase inhibitor--cerulenin inhibits proliferation of K562 cells but not of human fibroblasts. Cerulenin mediated cytotoxity is due to apoptosis induction. Fatty acid synthase might be a potential target for anti-leukemia. |
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| Keywords: | Cerulenin Cell line K562 Fatty acid synthase Apoptosis |
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