Effects of route and coadministration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice |
| |
Authors: | Brock Kingstad-Bakke Joseph N Brewoo Le Quynh Mai Yoshihiro Kawaoka Jorge E Osorio |
| |
Institution: | 1. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA;2. National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hanoi, Viet Nam;3. Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan;4. Department of Microbiology and Infectious Diseases, Kobe University, Hyogo 650-0017, Japan |
| |
Abstract: | We previously demonstrated that recombinant raccoonpox (RCN) virus could serve as a vector for an influenza vaccine. RCN constructs expressing the hemagglutinin (HA) from H5N1 viruses were immunogenic in chickens. In the current study, we generated several recombinant RCN constructs expressing influenza (H5N1) antigens and a molecular adjuvant (Heat-Labile enterotoxin B from E. coli: RCN-LTB), demonstrated their expression in vitro, and evaluated their ability to protect mice against H5N1 virus challenge. RCN-HA provided strong protection when administered intradermally (ID), but not intranasally (IN). Conversely, the RCN-neuraminidase (NA) construct was highly efficacious by the IN route and elicited high titers of neutralizing antibodies in mice. Vaccination by combined ID (RCN-HA) and IN (RCN-NA) routes offered mice the best protection against an IN challenge with heterologous H5N1 virus. However, protection was reduced when the different RCN constructs were pre-mixed, perhaps due to reduced expression of antigen. |
| |
Keywords: | Raccoon poxvirus HPAI Vaccine Mice Mucosal |
本文献已被 ScienceDirect 等数据库收录! |
|