庆大霉素-壳聚糖纳米粒治疗兔慢性骨髓炎的实验研究

目的:探讨庆大霉素-壳聚糖纳米粒(gentamicin loaded chitosan nanospheres,GS/CS NPs)对兔慢性胫骨骨髓炎的治疗作用?方法:取48只兔制作慢性胫骨骨髓炎模型,造模3周后行大体观察及患肢X线检查?44只兔造模成功后行不同治疗,A组:未特殊治疗;B组:患肢髓腔壳聚糖纳米粒填塞;C组:患肢髓腔庆大霉素粉末填塞;D组:患肢髓腔GS/CS NPs填塞?治疗3周后行患肢X线片检查及Norden评分?髓腔大体观及髓腔内容物细菌学培养,患肢标本行组织学观察及Smeltzer病理评分?结果:治疗3周后A?B?C组均出现明显的慢性骨髓炎X线表现,髓腔脓肿形成,髓腔内容物细菌学培养阳性,组织学观察示中性粒细胞浸润?骨小梁破坏,A?B组尚可见死骨形成,D组治疗后未见明显阳性表现?Norden评分D组较A?B?C组均有统计学意义(P < 0.05),Smeltzer病理评分D组较A?B?C组均有统计学意义(P < 0.05)?结论:庆大霉素-壳聚糖纳米粒作为局部释药系统治疗兔慢性骨髓炎具有良好的效果?

Study of treatment of chronic osteomyelitis of rabbit with gentamicin loaded chitosan nanospheres

Objective: To investigate the effect of gentamicin loaded chitosan nanospheres(GS/CS NPs) on the treatment of chronic osteomyelitis of rabbit. Methods: Forty-eight rabbits were used to establish the chronic osteomyelitis model,and observation of affected limb and X-ray were done 3 weeks later. Forty-four rabbits had symptoms and signs of chronic osteomyelitis and were divided into 4 groups. Group A has no treatment,group B was treated with chitosan nanospheres,group C was treated with gentamicin sulfate,and group D was treated with GS/CS NPs. The X-Ray,observation of the bone marrow cavity,bacterial culture and the HE staining were done after 3 weeks of treatment. Results: After 3 weeks of treatment,the X-Ray of groups A~C showed apparent signs of chronic osteomyelitis,the formation of abscess were observed in bone marrow cavity,and the cultures of the Staphylococcus aureus were positive. The HE staining of groups A~C showed neutrophil accumulation,and the formation of dead bone were observed in groups A~B. The Norden and Smeltzer scores showed significant differences between group D and groups A~C(both P < 0.05). Conclusion: GS/CS NPs has significant effects on the treatment of rabbit chronic osteomyelitis as local drug delivery system.