选择性抑制一氧化氮合酶对大鼠小体积肝移植的影响

目的:探讨诱导性一氧化氮合成酶(iNOS)在大鼠小体积肝移植模型中的作用?方法:选取清洁雄性Spraque-Dawley大鼠90只?采用改良的二袖套法建立吻合肝动脉的小移植物模型?实验分为3组:正常对照组?小体积肝移植组和氨基胍(AG)注射组?术后分别在1?3? 6?12 h等4个时间点处死5只大鼠并采取部分肝组织及血清样本?检测指标:血清丙氨酸转氨酶(ALT),实时荧光定量RT-PCR 测定肝组织iNOS mRNA 的表达,免疫组化法测定肝组织iNOS 蛋白的表达,HE染色光镜下病理观察等?结果:AG组与小体积肝移植组相比,实时荧光定量PCR显示:肝组织中iNOS mRNA 表达量明显下降(P < 0.05,n = 5);免疫组化显示:肝组织中iNOS 蛋白表达下降;但ALT 明显升高(P < 0.05,n = 5);病理显示肝细胞大量坏死?结论:AG 能抑制iNOS 在大鼠小体积模型中的表达,但不能减轻肝脏损伤,反而加重了损伤?

The role of selective inhibition of inducible nitric oxide synthase (iNOS) in small-for-size graft

Objective:To study the role of iNOS in small-for-size graft. Methods: A non-arterialized partial liver transplantation model was used. Rats were divided into three groups:control group,small-for-size group,Aminoguanidine (AG) group. The expression of the iNOS mRNA and protein,the level of alanine aminotransferase(ALT) in serum,as well as histology were checked after reperfusion. Results: The expression of the iNOS in AG group was lower than that of the small-for-size group,but the expression of ALT in serum sample was significantly higher than that of the small-for-size group(P < 0.05,n = 5). Histology showed necrosis was observed both in the small-for-size group and the AG group. Conclusion: AG can inhibit iNOS in small-for-size graft,but lead to the worse injury.